Influence of Selenium Content in the Culture Medium on Protein Profile of Yeast Cells Candida utilis ATCC 9950
Joint Authors
Kieliszek, Marek
Błażejak, Stanisław
Bzducha-Wróbel, Anna
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-21
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Selenium is an essential trace element for human health and it has been recognized as a component of several selenoproteins with crucial biological functions.
It has been identified as a component of active centers of many enzymes, as well as integral part of biologically active complexes.
The aim of the study was to evaluate the protein content and amino acid profile of the protein of fodder yeast Candida utilis ATCC 9950 cultured in media control and experimental enriched selenium.
Protein analysis was performed using SDS-PAGE method consisting of polyacrylamide gel electrophoresis in the presence of SDS.
The highest contents of soluble protein (49,5 mg/g) were found in yeast cells after 24-hour culture conducted in control (YPD) medium.
In the presence of selenium there were determined small amounts of protein content.
With increasing time of yeast culture (to 72 hours) the control and experimental media were reported to reduce soluble protein content.
In electropherogram proteins from control cultures was observed the presence of 10 protein fractions, but in all the experimental cultures (containing 20, 30, and 40 mg/L selenium) of 14 protein fractions.
On the basis of the molecular weights of proteins, it can be concluded that they were among others: selenoprotein 15 kDa and selenoprotein 18 kDa.
American Psychological Association (APA)
Kieliszek, Marek& Błażejak, Stanisław& Bzducha-Wróbel, Anna. 2015. Influence of Selenium Content in the Culture Medium on Protein Profile of Yeast Cells Candida utilis ATCC 9950. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1075730
Modern Language Association (MLA)
Kieliszek, Marek…[et al.]. Influence of Selenium Content in the Culture Medium on Protein Profile of Yeast Cells Candida utilis ATCC 9950. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1075730
American Medical Association (AMA)
Kieliszek, Marek& Błażejak, Stanisław& Bzducha-Wróbel, Anna. Influence of Selenium Content in the Culture Medium on Protein Profile of Yeast Cells Candida utilis ATCC 9950. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1075730
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075730