The Hydrogen Sulfide Donor NaHS Delays Programmed Cell Death in Barley Aleurone Layers by Acting as an Antioxidant
Joint Authors
Hu, Kang-Di
Hu, Lan-Ying
Zhang, Hua
Zhang, Ying-Xin
Lv, Kai
Li, Yan-Hong
Zhang, Xi-Qi
Ruan, Long
Liu, Yong-Sheng
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-11
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
H2S is a signaling molecule in plants and animals.
Here we investigated the effects of H2S on programmed cell death (PCD) in barley (Hordeum vulgare L.) aleurone layers.
The H2S donor NaHS significantly delayed PCD in aleurone layers isolated from imbibed embryoless barley grain.
NaHS at 0.25 mM effectively reduced the accumulation of superoxide anion (·O2-), hydrogen peroxide (H2O2), and malondialdehyde (MDA), promoted the activity of superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and decreased those of lipoxygenase (LOX) in isolated aleurone layers.
Quantitative-PCR showed that NaHS treatment of aleurone tissue led to enhanced transcript levels of the antioxidant genes HvSOD1, HvAPX, HvCAT1, and HvCAT2 and repressed transcript levels of HvLOX (lipoxygenase gene) and of two cysteine protease genes HvEPA and HvCP3-31.
NaHS treatment in gibberellic acid- (GA-) treated aleurone layers also delayed the PCD process, reduced the content of ·O2-, and increased POD activity while decreasing LOX activity.
Furthermore, α-amylase secretion in barley aleurone layers was enhanced by NaHS treatment regardless of the presence or absence of GA.
These data imply that H2S acted as an antioxidant in delaying PCD and enhances α-amylase secretion regardless of the presence of GA in barley aleurone layers.
American Psychological Association (APA)
Zhang, Ying-Xin& Hu, Kang-Di& Lv, Kai& Li, Yan-Hong& Hu, Lan-Ying& Zhang, Xi-Qi…[et al.]. 2015. The Hydrogen Sulfide Donor NaHS Delays Programmed Cell Death in Barley Aleurone Layers by Acting as an Antioxidant. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075742
Modern Language Association (MLA)
Zhang, Ying-Xin…[et al.]. The Hydrogen Sulfide Donor NaHS Delays Programmed Cell Death in Barley Aleurone Layers by Acting as an Antioxidant. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1075742
American Medical Association (AMA)
Zhang, Ying-Xin& Hu, Kang-Di& Lv, Kai& Li, Yan-Hong& Hu, Lan-Ying& Zhang, Xi-Qi…[et al.]. The Hydrogen Sulfide Donor NaHS Delays Programmed Cell Death in Barley Aleurone Layers by Acting as an Antioxidant. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075742
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075742