Lycopene Pretreatment Ameliorates Acute Ethanol Induced NAD+ Depletion in Human Astroglial Cells
Joint Authors
Guest, Jade
Heng, Benjamin
Grant, Ross
Guillemin, Gilles J.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Excessive alcohol consumption is associated with reduced brain volume and cognition.
While the mechanisms by which ethanol induces these deleterious effects in vivo are varied most are associated with increased inflammatory and oxidative processes.
In order to further characterise the effect of acute ethanol exposure on oxidative damage and NAD+ levels in the brain, human U251 astroglioma cells were exposed to physiologically relevant doses of ethanol (11 mM, 22 mM, 65 mM, and 100 mM) for ≤ 30 minutes.
Ethanol exposure resulted in a dose dependent increase in both ROS and poly(ADP-ribose) polymer production.
Significant decreases in total NAD(H) and sirtuin 1 activity were also observed at concentrations ≥ 22 mM.
Similar to U251 cells, exposure to ethanol (≥22 mM) decreased levels of NAD(H) in primary human astrocytes.
NAD(H) depletion in primary astrocytes was prevented by pretreatment with 1 μM of lycopene for 3.5 hours.
Unexpectedly, in U251 cells lycopene treatment at concentrations ≥ 5 μM resulted in significant reductions in [NAD(H)].
This study suggests that exposure of the brain to alcohol at commonly observed blood concentrations may cause transitory oxidative damage which may be at least partly ameliorated by lycopene.
American Psychological Association (APA)
Guest, Jade& Guillemin, Gilles J.& Heng, Benjamin& Grant, Ross. 2015. Lycopene Pretreatment Ameliorates Acute Ethanol Induced NAD+ Depletion in Human Astroglial Cells. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1075755
Modern Language Association (MLA)
Guest, Jade…[et al.]. Lycopene Pretreatment Ameliorates Acute Ethanol Induced NAD+ Depletion in Human Astroglial Cells. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1075755
American Medical Association (AMA)
Guest, Jade& Guillemin, Gilles J.& Heng, Benjamin& Grant, Ross. Lycopene Pretreatment Ameliorates Acute Ethanol Induced NAD+ Depletion in Human Astroglial Cells. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1075755
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075755