Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro
Joint Authors
Djelic, Ninoslav
Živković, Lada
Žukovec Topalović, Dijana
Čabarkapa, Andrea
Bajić, Vladan
Dekanski, Dragana
Spremo-Potparević, Biljana
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks.
Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants.
The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay.
Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment.
Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity.
The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P < 0.05).
Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect.
This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.
American Psychological Association (APA)
Žukovec Topalović, Dijana& Živković, Lada& Čabarkapa, Andrea& Djelic, Ninoslav& Bajić, Vladan& Dekanski, Dragana…[et al.]. 2015. Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1075763
Modern Language Association (MLA)
Žukovec Topalović, Dijana…[et al.]. Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1075763
American Medical Association (AMA)
Žukovec Topalović, Dijana& Živković, Lada& Čabarkapa, Andrea& Djelic, Ninoslav& Bajić, Vladan& Dekanski, Dragana…[et al.]. Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1075763
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075763