GC-Rich Extracellular DNA Induces Oxidative Stress, Double-Strand DNA Breaks, and DNA Damage Response in Human Adipose-Derived Mesenchymal Stem Cells
Joint Authors
Izevskaya, Vera L.
Kostyuk, Svetlana
Smirnova, Tatiana
Kameneva, Larisa
Porokhovnik, Lev
Speranskij, Anatolij
Ershova, Elizaveta
Stukalov, Sergey
Veiko, Natalia
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-26
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Background.
Cell free DNA (cfDNA) circulates throughout the bloodstream of both healthy people and patients with various diseases.
CfDNA is substantially enriched in its GC-content as compared with human genomic DNA.
Principal Findings.
Exposure of haMSCs to GC-DNA induces short-term oxidative stress (determined with H2DCFH-DA) and results in both single- and double-strand DNA breaks (comet assay and γH2AX, foci).
As a result in the cells significantly increases the expression of repair genes (BRCA1 (RT-PCR), PCNA (FACS)) and antiapoptotic genes (BCL2 (RT-PCR and FACS), BCL2A1, BCL2L1, BIRC3, and BIRC2 (RT-PCR)).
Under the action of GC-DNA the potential of mitochondria was increased.
Here we show that GC-rich extracellular DNA stimulates adipocyte differentiation of human adipose-derived mesenchymal stem cells (haMSCs).
Exposure to GC-DNA leads to an increase in the level of RNAPPARG2 and LPL (RT-PCR), in the level of fatty acid binding protein FABP4 (FACS analysis) and in the level of fat (Oil Red O).
Conclusions.
GC-rich fragments in the pool of cfDNA can potentially induce oxidative stress and DNA damage response and affect the direction of mesenchymal stem cells differentiation in human adipose—derived mesenchymal stem cells.
Such a response may be one of the causes of obesity or osteoporosis.
American Psychological Association (APA)
Kostyuk, Svetlana& Smirnova, Tatiana& Kameneva, Larisa& Porokhovnik, Lev& Speranskij, Anatolij& Ershova, Elizaveta…[et al.]. 2015. GC-Rich Extracellular DNA Induces Oxidative Stress, Double-Strand DNA Breaks, and DNA Damage Response in Human Adipose-Derived Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1075767
Modern Language Association (MLA)
Kostyuk, Svetlana…[et al.]. GC-Rich Extracellular DNA Induces Oxidative Stress, Double-Strand DNA Breaks, and DNA Damage Response in Human Adipose-Derived Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-15.
https://search.emarefa.net/detail/BIM-1075767
American Medical Association (AMA)
Kostyuk, Svetlana& Smirnova, Tatiana& Kameneva, Larisa& Porokhovnik, Lev& Speranskij, Anatolij& Ershova, Elizaveta…[et al.]. GC-Rich Extracellular DNA Induces Oxidative Stress, Double-Strand DNA Breaks, and DNA Damage Response in Human Adipose-Derived Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1075767
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075767