Upregulation of Multidrug Resistance-Associated Protein 1 by Allyl Isothiocyanate in Human Bronchial Epithelial Cell: Involvement of c-Jun N-Terminal Kinase Signaling Pathway
Joint Authors
Wang, Chen-yin
Wang, Shan-shan
Li, Ze-geng
Wang, Shujun
Chen, Yajun
Li, Jie
Wang, Xueqi
Wang, Dianlei
Peng, Zhaoliang
Fan, Ling
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Multidrug resistance-associated protein 1 (MRP1) plays a protective role in the etiology and progression of chronic obstructive pulmonary disease (COPD) which results from oxidative stress and inflammation of lung injury.
The lower functional MRP1 activity is related to COPD development.
Our previous study showed that Allyl isothiocyanate (AITC) induced the expression and activity of MRP1 in a dose-dependent manner.
However, which signaling pathway contributes to the upregulation of MRP1 by AITC is unclear.
In this study, signaling pathway specific inhibitors were used to examine the mechanism of AITC.
We found that JNK inhibitor SP600125 treatment decreased MRP1 mRNA expression in 16HBE14o- cells.
But the ERK inhibitor U0126 or PI3K/Akt inhibitor LY294002 produced no obvious effect.
The AITC-induced increase of MRP1 mRNA expression was abolished by cotreatment of SP600125, while it was not obviously affected by U0126 or LY294002.
Furthermore, AITC acivates the JNK signaling pathway in 16HBE14o- cells.
Finally, we found that JNK pathway mediated the upregulation of AITC-induced expression and function of MRP1.
Taken together, our results indicated that AITC increased the expression and the activity of MRP1 via a JNK-dependent pathway.
ERK and PI3K signaling pathway were not involved in the expression of MRP1 mRNA.
American Psychological Association (APA)
Wang, Shujun& Wang, Shan-shan& Wang, Chen-yin& Chen, Yajun& Li, Jie& Wang, Xueqi…[et al.]. 2015. Upregulation of Multidrug Resistance-Associated Protein 1 by Allyl Isothiocyanate in Human Bronchial Epithelial Cell: Involvement of c-Jun N-Terminal Kinase Signaling Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1075806
Modern Language Association (MLA)
Wang, Shujun…[et al.]. Upregulation of Multidrug Resistance-Associated Protein 1 by Allyl Isothiocyanate in Human Bronchial Epithelial Cell: Involvement of c-Jun N-Terminal Kinase Signaling Pathway. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1075806
American Medical Association (AMA)
Wang, Shujun& Wang, Shan-shan& Wang, Chen-yin& Chen, Yajun& Li, Jie& Wang, Xueqi…[et al.]. Upregulation of Multidrug Resistance-Associated Protein 1 by Allyl Isothiocyanate in Human Bronchial Epithelial Cell: Involvement of c-Jun N-Terminal Kinase Signaling Pathway. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1075806
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075806