Ginkgolide B Inhibits JAM-A, Cx43, and VE-Cadherin Expression and Reduces Monocyte Transmigration in Oxidized LDL-Stimulated Human Umbilical Vein Endothelial Cells
Joint Authors
Liu, Xueqing
Sun, Wenjia
Zhao, Yanyang
Chen, Beidong
Wu, Wei
Bao, Li
Qi, Ruomei
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Aim.
To investigate the effect of ginkgolide B on junction proteins and the reduction of monocyte migration in oxidized low-density lipoprotein- (ox-LDL-) treated endothelial cells.
Methods.
Human umbilical vein endothelial cells (HUVECs) were used in the present study.
Immunofluorescence and Western blot were performed to determine the expression of junctional adhesion molecule-A (JAM-A), connexin 43 (Cx43), and vascular endothelial cadherin (VE-cadherin).
Monocyte migration was detected by the Transwell assay.
Results.
ox-LDL stimulation increased JAM-A expression by 35%, Cx43 expression by 24%, and VE-cadherin expression by 37% in HUVECs.
Ginkgolide B (0.2, 0.4, and 0.6 mg/mL) dose-dependently abolished the expression of these junction proteins.
The monocyte transmigration experiments showed that the level of monocyte migration was sixfold higher in the ox-LDL-treated group than in the control group.
Ginkgolide B (0.6 mg/mL) nearly completely abolished monocyte migration.
Both ginkgolide B and LY294002 suppressed Akt phosphorylation and the expression of these junction proteins in ox-LDL-treated endothelial cells.
These results suggest that the ginkgolide B-induced inhibition of junction protein expression is associated with blockade of the PI3K/Akt pathway.
Conclusion.
Ginkgolide B suppressed junction protein expression and reduced monocyte transmigration that was induced by ox-LDL.
Ginkgolide B may improve vascular permeability in atherosclerosis.
American Psychological Association (APA)
Liu, Xueqing& Sun, Wenjia& Zhao, Yanyang& Chen, Beidong& Wu, Wei& Bao, Li…[et al.]. 2015. Ginkgolide B Inhibits JAM-A, Cx43, and VE-Cadherin Expression and Reduces Monocyte Transmigration in Oxidized LDL-Stimulated Human Umbilical Vein Endothelial Cells. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075808
Modern Language Association (MLA)
Liu, Xueqing…[et al.]. Ginkgolide B Inhibits JAM-A, Cx43, and VE-Cadherin Expression and Reduces Monocyte Transmigration in Oxidized LDL-Stimulated Human Umbilical Vein Endothelial Cells. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1075808
American Medical Association (AMA)
Liu, Xueqing& Sun, Wenjia& Zhao, Yanyang& Chen, Beidong& Wu, Wei& Bao, Li…[et al.]. Ginkgolide B Inhibits JAM-A, Cx43, and VE-Cadherin Expression and Reduces Monocyte Transmigration in Oxidized LDL-Stimulated Human Umbilical Vein Endothelial Cells. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075808
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075808