Sesquiterpene Lactones Inhibit Advanced Oxidation Protein Product-Induced MCP-1 Expression in Podocytes via an IKKNF-κB-Dependent Mechanism
Joint Authors
Zhao, Yan
Chen, Si-jia
Wang, Jian-cheng
Niu, Hong-xin
Jia, Qian-qian
Chen, Xiao-wen
Du, Xiao-yan
Lu, Lu
Huang, Bo
Zhang, Quan
Chen, Yue
Long, Hai-bo
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-12
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Inflammation is a relevant factor in the pathogenesis of diabetes nephropathy (DN).
Sesquiterpene lactones (SLs), originally isolated from Tanacetum parthenium, have been reported to exhibit anti-inflammatory effects but few studies have examined their effects on DN.
To determine whether advanced oxidation protein products (AOPPs) can induce the expression of chemokine monocyte chemoattractant protein- (MCP-) 1 in cultured mouse podocytes and to explore the mechanisms of the potential renoprotection of SLs, we treated podocytes with AOPPs and SLs (parthenolide and its derivatives micheliolide, compound 1, and compound 2).
MCP-1 mRNA and protein expression were tested using quantitative real-time PCR and ELISA, respectively, and the protein levels of IKKβ, phospho-IKKβ, IκBα, NF-κB p65, phospho-NF-κB p65, and tubulin were analyzed by Western blotting.
AOPPs activated the expression of MCP-1 mRNA and protein in a dose- and time-dependent manner, activated IKKβ and NF-κB p65, and promoted IκBα degradation.
The IKK/NF-κB inhibitor parthenolide decreased AOPP-induced MCP-1 expression.
Pretreatment with SLs inhibited MCP-1 mRNA and protein expression and suppressed IKKβ and NF-κB p65 phosphorylation and IκBα degradation.
Taken together, these findings provide a novel explanation for the anti-inflammatory effects of SLs that will ultimately benefit DN and potentially other inflammatory and immune renal diseases.
American Psychological Association (APA)
Zhao, Yan& Chen, Si-jia& Wang, Jian-cheng& Niu, Hong-xin& Jia, Qian-qian& Chen, Xiao-wen…[et al.]. 2015. Sesquiterpene Lactones Inhibit Advanced Oxidation Protein Product-Induced MCP-1 Expression in Podocytes via an IKKNF-κB-Dependent Mechanism. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1075815
Modern Language Association (MLA)
Zhao, Yan…[et al.]. Sesquiterpene Lactones Inhibit Advanced Oxidation Protein Product-Induced MCP-1 Expression in Podocytes via an IKKNF-κB-Dependent Mechanism. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1075815
American Medical Association (AMA)
Zhao, Yan& Chen, Si-jia& Wang, Jian-cheng& Niu, Hong-xin& Jia, Qian-qian& Chen, Xiao-wen…[et al.]. Sesquiterpene Lactones Inhibit Advanced Oxidation Protein Product-Induced MCP-1 Expression in Podocytes via an IKKNF-κB-Dependent Mechanism. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1075815
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075815