Hydrogen Sulfide Prevents Advanced Glycation End-Products Induced Activation of the Epithelial Sodium Channel
Joint Authors
Wang, Qiushi
Song, Binlin
Jiang, Shuai
Liang, Chen
Chen, Xiao
Shi, Jing
Li, Xinyuan
Sun, Yingying
Wu, Mingming
Zhao, Dan
Zhang, Zhi-Ren
Ma, He-Ping
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-11
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Advanced glycation end-products (AGEs) are complex and heterogeneous compounds implicated in diabetes.
Sodium reabsorption through the epithelial sodium channel (ENaC) at the distal nephron plays an important role in diabetic hypertension.
Here, we report that H2S antagonizes AGEs-induced ENaC activation in A6 cells.
ENaC open probability (PO) in A6 cells was significantly increased by exogenous AGEs and that this AGEs-induced ENaC activity was abolished by NaHS (a donor of H2S) and TEMPOL.
Incubating A6 cells with the catalase inhibitor 3-aminotriazole (3-AT) mimicked the effects of AGEs on ENaC activity, but did not induce any additive effect.
We found that the expression levels of catalase were significantly reduced by AGEs and both AGEs and 3-AT facilitated ROS uptake in A6 cells, which were significantly inhibited by NaHS.
The specific PTEN and PI3K inhibitors, BPV(pic) and LY294002, influence ENaC activity in AGEs-pretreated A6 cells.
Moreover, after removal of AGEs from AGEs-pretreated A6 cells for 72 hours, ENaC PO remained at a high level, suggesting that an AGEs-related “metabolic memory” may be involved in sodium homeostasis.
Our data, for the first time, show that H2S prevents AGEs-induced ENaC activation by targeting the ROS/PI3K/PTEN pathway.
American Psychological Association (APA)
Wang, Qiushi& Song, Binlin& Jiang, Shuai& Liang, Chen& Chen, Xiao& Shi, Jing…[et al.]. 2015. Hydrogen Sulfide Prevents Advanced Glycation End-Products Induced Activation of the Epithelial Sodium Channel. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075827
Modern Language Association (MLA)
Wang, Qiushi…[et al.]. Hydrogen Sulfide Prevents Advanced Glycation End-Products Induced Activation of the Epithelial Sodium Channel. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1075827
American Medical Association (AMA)
Wang, Qiushi& Song, Binlin& Jiang, Shuai& Liang, Chen& Chen, Xiao& Shi, Jing…[et al.]. Hydrogen Sulfide Prevents Advanced Glycation End-Products Induced Activation of the Epithelial Sodium Channel. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075827
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075827