Smad23 Upregulates the Expression of Vimentin and Affects Its Distribution in DBP-Exposed Sertoli Cells
Joint Authors
Cui, Zhihong
Liu, Jing-yi
Cao, Jia
Ao, Lin
Zhang, Xi
Wang, Xiaogang
Liu, Taixiu
Mo, Min
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Sertoli cells (SCs) in the testes provide physical and nutritional support to germ cells.
The vimentin cytoskeleton in SCs is disrupted by dibutyl phthalate (DBP), which leads to SCs dysfunction.
In a previous study, we found that peroxisome proliferator-activated receptor alpha (PPARα) influenced the distribution of vimentin by affecting its phosphorylation in DBP-exposed SCs.
In the present study, we investigated the role of Smad2/3 in regulating the expression of vimentin in DBP-exposed SCs.
We hypothesized that Smad2/3 affects the distribution of vimentin by regulating its expression and that there is cross talk between Smad2/3 and PPARα.
The real-time PCR and ChIP-qPCR results showed that SB431542 (an inhibitor of Smad2/3) could significantly attenuate the expression of vimentin induced by DBP in SCs.
Phosphorylated and soluble vimentin were both downregulated by SB431542 pretreatment.
WY14643 (an agonist of PPARα) pretreatment stimulated, while GW6471 (an antagonist of PPARα) inhibited, the activity of Smad2/3; SB431542 pretreatment also inhibited the activity of PPARα, but it did not rescue the DBP-induced collapse in vimentin.
Our results suggest that, in addition to promoting the phosphorylation of vimentin, DBP also stimulates the expression of vimentin by activating Smad2/3 in SCs and thereby induces irregular vimentin distribution.
American Psychological Association (APA)
Zhang, Xi& Wang, Xiaogang& Liu, Taixiu& Mo, Min& Ao, Lin& Liu, Jing-yi…[et al.]. 2015. Smad23 Upregulates the Expression of Vimentin and Affects Its Distribution in DBP-Exposed Sertoli Cells. PPAR Research،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075962
Modern Language Association (MLA)
Zhang, Xi…[et al.]. Smad23 Upregulates the Expression of Vimentin and Affects Its Distribution in DBP-Exposed Sertoli Cells. PPAR Research No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1075962
American Medical Association (AMA)
Zhang, Xi& Wang, Xiaogang& Liu, Taixiu& Mo, Min& Ao, Lin& Liu, Jing-yi…[et al.]. Smad23 Upregulates the Expression of Vimentin and Affects Its Distribution in DBP-Exposed Sertoli Cells. PPAR Research. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075962
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075962