![](/images/graphics-bg.png)
AICAR Protects against High PalmitateHigh Insulin-Induced Intramyocellular Lipid Accumulation and Insulin Resistance in HL-1 Cardiac Cells by Inducing PPAR-Target Gene Expression
Joint Authors
Rodríguez-Calvo, Ricardo
Masana, Luis
Neumann, Dietbert
Vázquez-Carrera, Manuel
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Here we studied the impact of 5-aminoimidazole-4-carboxamide riboside (AICAR), a well-known AMPK activator, on cardiac metabolic adaptation.
AMPK activation by AICAR was confirmed by increased phospho-Thr172-AMPK and phospho-Ser79-ACC protein levels in HL-1 cardiomyocytes.
Then, cells were exposed to AICAR stimulation for 24 h in the presence or absence of the AMPK inhibitor Compound C, and the mRNA levels of the three PPARs were analyzed by real-time RT-PCR.
Treatment with AICAR induced gene expression of all three PPARs, but only the Ppara and Pparg regulation were dependent on AMPK.
Next, we exposed HL-1 cells to high palmitate/high insulin (HP/HI) conditions either in presence or in absence of AICAR, and we evaluated the expression of selected PPAR-targets genes.
HP/HI induced insulin resistance and lipid storage was accompanied by increased Cd36, Acot1, and Ucp3 mRNA levels.
AICAR treatment induced the expression of Acadvl and Glut4, which correlated to prevention of the HP/HI-induced intramyocellular lipid build-up, and attenuation of the HP/HI-induced impairment of glucose uptake.
These data support the hypothesis that AICAR contributes to cardiac metabolic adaptation via regulation of transcriptional mechanisms.
American Psychological Association (APA)
Rodríguez-Calvo, Ricardo& Vázquez-Carrera, Manuel& Masana, Luis& Neumann, Dietbert. 2015. AICAR Protects against High PalmitateHigh Insulin-Induced Intramyocellular Lipid Accumulation and Insulin Resistance in HL-1 Cardiac Cells by Inducing PPAR-Target Gene Expression. PPAR Research،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1075968
Modern Language Association (MLA)
Rodríguez-Calvo, Ricardo…[et al.]. AICAR Protects against High PalmitateHigh Insulin-Induced Intramyocellular Lipid Accumulation and Insulin Resistance in HL-1 Cardiac Cells by Inducing PPAR-Target Gene Expression. PPAR Research No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1075968
American Medical Association (AMA)
Rodríguez-Calvo, Ricardo& Vázquez-Carrera, Manuel& Masana, Luis& Neumann, Dietbert. AICAR Protects against High PalmitateHigh Insulin-Induced Intramyocellular Lipid Accumulation and Insulin Resistance in HL-1 Cardiac Cells by Inducing PPAR-Target Gene Expression. PPAR Research. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1075968
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075968