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Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures
Joint Authors
Somasundaram, Indumathi
Mishra, Rashmi
Radhakrishnan, Harikrishnan
Sankaran, Rajkumar
Garikipati, Venkata Naga Srikanth
Marappagounder, Dhanasekaran
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-29, 29 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-20
Country of Publication
Egypt
No. of Pages
29
Abstract EN
The study aims to identify the phenotypic marker expressions of different human adult stem cells derived from, namely, bone marrow, subcutaneous fat, and omentum fat, cultured in different media, namely, DMEM-Low Glucose, Alpha-MEM, DMEM-F12 and DMEM-KO and under long term culture conditions (>P20).
We characterized immunophenotype by using various hematopoietic, mesenchymal, endothelial markers, and cell adhesion molecules in the long term cultures (Passages-P1, P3, P5, P9, P12, P15, and P20.) Interestingly, data revealed similar marker expression profiles irrespective of source, basal media, and extensive culturing.
This demonstrates that all adult stem cell sources mentioned in this study share similar phenotypic marker and all media seem appropriate for culturing these sources.
However, a disparity was observed in the markers such as CD49d, CD54, CD117, CD29, and CD106, thereby warranting further research on these markers.
Besides the aforesaid objective, it is understood from the study that immunophenotyping acts as a valuable tool to identify inherent property of each cell, thereby leading to a valuable cell based therapy.
American Psychological Association (APA)
Somasundaram, Indumathi& Mishra, Rashmi& Radhakrishnan, Harikrishnan& Sankaran, Rajkumar& Garikipati, Venkata Naga Srikanth& Marappagounder, Dhanasekaran. 2015. Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures. Stem Cells International،Vol. 2015, no. 2015, pp.1-29.
https://search.emarefa.net/detail/BIM-1076129
Modern Language Association (MLA)
Somasundaram, Indumathi…[et al.]. Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures. Stem Cells International No. 2015 (2015), pp.1-29.
https://search.emarefa.net/detail/BIM-1076129
American Medical Association (AMA)
Somasundaram, Indumathi& Mishra, Rashmi& Radhakrishnan, Harikrishnan& Sankaran, Rajkumar& Garikipati, Venkata Naga Srikanth& Marappagounder, Dhanasekaran. Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-29.
https://search.emarefa.net/detail/BIM-1076129
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076129