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Selection of Reference Genes for Quantitative Gene Expression in Porcine Mesenchymal Stem Cells Derived from Various Sources along with Differentiation into Multilineages
Joint Authors
Lee, Won-Jae
Jeon, Ryoung-Hoon
Jang, Si-Jung
Park, Ji-Sung
Lee, Seung-Chan
Baregundi Subbarao, Raghavendra
Lee, Sung-Lim
Park, Bong-Wook
Rho, Gyu-Jin
King, W. A.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-20
Country of Publication
Egypt
No. of Pages
14
Abstract EN
The identification of stable reference genes is a prerequisite for ensuring accurate validation of gene expression, yet too little is known about stable reference genes of porcine MSCs.
The present study was, therefore, conducted to assess the stability of reference genes in porcine MSCs derived from bone marrow (BMSCs), adipose (AMSCs), and skin (SMSCs) with their in vitro differentiated cells into mesenchymal lineages such as adipocytes, osteocytes, and chondrocytes.
Twelve commonly used reference genes were investigated for their threshold cycle (Ct) values by qRT-PCR.
The Ct values of candidate reference genes were analyzed by geNorm software to clarify stable expression regardless of experimental conditions.
Thus, Pearson’s correlation was applied to determine correlation between the three most stable reference genes (NF3) and optimal number of reference genes (NFopt).
In assessment of stability of reference gene across experimental conditions by geNorm analysis, undifferentiated MSCs and each differentiated status into mesenchymal lineages showed slightly different results but similar patterns about more or less stable rankings.
Furthermore, Pearson’s correlation revealed high correlation (r>0.9) between NF3 and NFopt.
Overall, the present study showed that HMBS, YWHAZ, SDHA, and TBP are suitable reference genes for qRT-PCR in porcine MSCs.
American Psychological Association (APA)
Lee, Won-Jae& Jeon, Ryoung-Hoon& Jang, Si-Jung& Park, Ji-Sung& Lee, Seung-Chan& Baregundi Subbarao, Raghavendra…[et al.]. 2015. Selection of Reference Genes for Quantitative Gene Expression in Porcine Mesenchymal Stem Cells Derived from Various Sources along with Differentiation into Multilineages. Stem Cells International،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1076162
Modern Language Association (MLA)
Lee, Won-Jae…[et al.]. Selection of Reference Genes for Quantitative Gene Expression in Porcine Mesenchymal Stem Cells Derived from Various Sources along with Differentiation into Multilineages. Stem Cells International No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1076162
American Medical Association (AMA)
Lee, Won-Jae& Jeon, Ryoung-Hoon& Jang, Si-Jung& Park, Ji-Sung& Lee, Seung-Chan& Baregundi Subbarao, Raghavendra…[et al.]. Selection of Reference Genes for Quantitative Gene Expression in Porcine Mesenchymal Stem Cells Derived from Various Sources along with Differentiation into Multilineages. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1076162
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076162