A 3D Porous Gelatin-Alginate-Based-IPN Acts as an Efficient Promoter of Chondrogenesis from Human Adipose-Derived Stem Cells
Joint Authors
Stancu, Izabela-Cristina
Lungu, Adriana
Hermenean, Anca
Jianu, Dana
Tumbar, Tudorita
Galateanu, Bianca
Dinescu, Sorina
Marieta, Costache
Radu, Eugen
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-27
Country of Publication
Egypt
No. of Pages
17
Abstract EN
Cartilage has limited regeneration potential.
Thus, there is an imperative need to develop new strategies for cartilage tissue engineering (CTE) amenable for clinical use.
Recent CTE approaches rely on optimal cell-scaffold interactions, which require a great deal of optimization.
In this study we attempt to build a novel gelatin- (G-) alginate- (A-) polyacrylamide (PAA) 3D interpenetrating network (IPN) with superior performance in promoting chondrogenesis from human adipose-derived stem cells (hADSCs).
We show that our G-A-PAA scaffold is capable of supporting hADSCs proliferation and survival, with no apparent cytotoxic effect.
Moreover, we find that after exposure to prochondrogenic conditions a key transcription factor known to induce chondrogenesis, namely, Sox9, is highly expressed in our hADSCs/G-A-PAA bioconstruct, along with cartilage specific markers such as collagen type II, CEP68, and COMP extracellular matrix (ECM) components.
These data suggest that our G-A-PAA structural properties and formulation might enable hADSCs conversion towards functional chondrocytes.
We conclude that our novel G-A-PAA biomatrix is a good candidate for prospective in vivo CTE applications.
American Psychological Association (APA)
Dinescu, Sorina& Galateanu, Bianca& Radu, Eugen& Hermenean, Anca& Lungu, Adriana& Stancu, Izabela-Cristina…[et al.]. 2015. A 3D Porous Gelatin-Alginate-Based-IPN Acts as an Efficient Promoter of Chondrogenesis from Human Adipose-Derived Stem Cells. Stem Cells International،Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1076174
Modern Language Association (MLA)
Dinescu, Sorina…[et al.]. A 3D Porous Gelatin-Alginate-Based-IPN Acts as an Efficient Promoter of Chondrogenesis from Human Adipose-Derived Stem Cells. Stem Cells International No. 2015 (2015), pp.1-17.
https://search.emarefa.net/detail/BIM-1076174
American Medical Association (AMA)
Dinescu, Sorina& Galateanu, Bianca& Radu, Eugen& Hermenean, Anca& Lungu, Adriana& Stancu, Izabela-Cristina…[et al.]. A 3D Porous Gelatin-Alginate-Based-IPN Acts as an Efficient Promoter of Chondrogenesis from Human Adipose-Derived Stem Cells. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1076174
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076174