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Role of Bone Marrow-Derived Stem Cells in Polyps Development in Mice with ApcMin+ Mutation
Joint Authors
Licinio, Raffaele
Di Leo, Alfredo
Barone, Michele
Scavo, Maria Principia
Piombino, Michele
De Tullio, Nicola
Mallamaci, Rosanna
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-16
Country of Publication
Egypt
No. of Pages
7
Abstract EN
We explored the hypothesis that an altered microenvironment (intestinal adenomatous polyp) could modify the differentiation program of bone marrow-derived stem cells (BMSCs), involving them in colon carcinogenesis.
Sublethally irradiated 8-week-old female ApcMin/+ mice were transplanted with bone marrow (BM) cells obtained from either male age-matched ApcMin/+ (Apc-Tx-Apc) or wild type (WT) (WT-Tx-Apc) mice.
At 4 and 7 weeks after transplantation, BM-derived colonocytes were recognized by colocalization of Y-chromosome and Cdx2 protein (specific colonocyte marker).
Polyp number, volume, and grade of dysplasia were not influenced by irradiation/transplantation procedures since they were similar in both untreated female ApcMin/+ and Apc-Tx-Apc mice.
At 4 and 7 weeks after transplantation, a progressive significant reduction of polyp number and volume was observed in WT-Tx-Apc mice.
Moreover, the number of WT-Tx-Apc mice with a high-grade dysplastic polyps significantly decreased as compared to Apc-Tx-Apc mice.
Finally, at 4 and 7 weeks after transplantation, WT-Tx-Apc mice showed a progressive significant increase of Y+/Cdx2+ cells in “normal” mucosa, whereas, in the adenomatous tissue, Y+/Cdx2+ cells remained substantially unvaried.
Our findings demonstrate that WT BMSCs do not participate in polyp development but rather inhibit their growth.
The substitution of genotypically altered colonocytes with Y+/Cdx2+ cells probably contributes to this process.
American Psychological Association (APA)
Barone, Michele& Scavo, Maria Principia& Licinio, Raffaele& Piombino, Michele& De Tullio, Nicola& Mallamaci, Rosanna…[et al.]. 2015. Role of Bone Marrow-Derived Stem Cells in Polyps Development in Mice with ApcMin+ Mutation. Stem Cells International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1076190
Modern Language Association (MLA)
Barone, Michele…[et al.]. Role of Bone Marrow-Derived Stem Cells in Polyps Development in Mice with ApcMin+ Mutation. Stem Cells International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1076190
American Medical Association (AMA)
Barone, Michele& Scavo, Maria Principia& Licinio, Raffaele& Piombino, Michele& De Tullio, Nicola& Mallamaci, Rosanna…[et al.]. Role of Bone Marrow-Derived Stem Cells in Polyps Development in Mice with ApcMin+ Mutation. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1076190
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076190