Effect of Increasing Doses of γ-Radiation on Bone Marrow Stromal Cells Grown on Smooth and Rough Titanium Surfaces
Joint Authors
Huang, Bo
Guang, Mengkai
Ye, Jun
Gong, Ping
Tang, Hua
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-15
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Radiation therapy for oral and maxillofacial tumors could damage bone marrow stromal cells (BMSCs) in jaw, which caused dental implant failure.
However, how radiation affects BMSCs on SLA (sandblasted with large-grits, acid-etched) surfaces is still unknown.
The aim of this study was to investigate effect of different dose of γ-radiation on BMSCs on SLA and PT (polished titanium) surfaces.
Rat BMSCs were radiated with 2, 4, and 8 Gy γ-radiation and then seeded on both surfaces.
Cell adhesion, spreading, and proliferation were tested.
The osteogenesis and the adipogenesis ability were examined by Alizarin-Red and Oil-Red staining, respectively.
Real-time PCR was performed to detect osteogenic (osteocalcin, OCN; runt-related transcription factor 2, Runx2) and adipogenic (peroxisome proliferator-activated receptor gamma, PPARγ) gene expression at days 7 and 14 postirradiation.
Results showed that γ-radiation reduced cell proliferation, adhesion, spreading, and osteogenic differentiation.
2 Gy radiation promoted adipogenic differentiation, but it was significantly decreased when dosage reached 4 Gy.
In conclusion, results suggest that γ-radiation influenced BMSCs behaviors in a dosage-dependent manner except adipogenic differentiation, low dose promoted it, and high dose inhibited it.
This effect was influenced by surface characteristics, which may explain the different failure rate of various implants in patients after radiation.
American Psychological Association (APA)
Huang, Bo& Guang, Mengkai& Ye, Jun& Gong, Ping& Tang, Hua. 2015. Effect of Increasing Doses of γ-Radiation on Bone Marrow Stromal Cells Grown on Smooth and Rough Titanium Surfaces. Stem Cells International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1076191
Modern Language Association (MLA)
Huang, Bo…[et al.]. Effect of Increasing Doses of γ-Radiation on Bone Marrow Stromal Cells Grown on Smooth and Rough Titanium Surfaces. Stem Cells International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1076191
American Medical Association (AMA)
Huang, Bo& Guang, Mengkai& Ye, Jun& Gong, Ping& Tang, Hua. Effect of Increasing Doses of γ-Radiation on Bone Marrow Stromal Cells Grown on Smooth and Rough Titanium Surfaces. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1076191
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076191