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High OCT4 and Low p16INK4A Expressions Determine In Vitro Lifespan of Mesenchymal Stem Cells
Joint Authors
Piccinato, Carla A.
Sertie, Andrea L.
Torres, Natália
Ferretti, Mario
Antonioli, Eliane
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-21
Country of Publication
Egypt
No. of Pages
11
Abstract EN
After long-term culture, mesenchymal stem cells alter their biological properties and enter into a state of replicative senescence.
Although several classical biomarkers have been used for quantitative assessment of cellular senescence, no hallmark has been proven completely unique to the senescent state in cells.
We used bone marrow-derived MSCs (BM-MSCs) from different healthy young donors and an in vitro model with well-defined senescence end points to identify a set of robust markers that could potentially predict the expansion capacity of MSCs preparations before reaching senescence.
For each early passage BM-MSC sample (5th or 6th passages), the normalized protein expression levels of senescence-associated markers p16INK4A, p21WAF1, SOD2, and rpS6S240/244; the concentration of IL6 and IL8 in cell culture supernatants; and the normalized gene expression levels of pluripotency markers OCT4, NANOG, and SOX2 were correlated with final population doubling (PD) number.
We revealed that the low expression of p16INK4A protein and a high OCT4 gene expression, rather than other evaluated markers, might be potential hallmarks and predictors of greater in vitro lifespan and growth potential, factors that can impact the successful therapeutic use of MSCs preparations.
American Psychological Association (APA)
Piccinato, Carla A.& Sertie, Andrea L.& Torres, Natália& Ferretti, Mario& Antonioli, Eliane. 2015. High OCT4 and Low p16INK4A Expressions Determine In Vitro Lifespan of Mesenchymal Stem Cells. Stem Cells International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1076194
Modern Language Association (MLA)
Piccinato, Carla A.…[et al.]. High OCT4 and Low p16INK4A Expressions Determine In Vitro Lifespan of Mesenchymal Stem Cells. Stem Cells International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1076194
American Medical Association (AMA)
Piccinato, Carla A.& Sertie, Andrea L.& Torres, Natália& Ferretti, Mario& Antonioli, Eliane. High OCT4 and Low p16INK4A Expressions Determine In Vitro Lifespan of Mesenchymal Stem Cells. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1076194
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076194