Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4
Joint Authors
Liu, Zhijing
Lu, Yan
Tan, Xiaohua
Zhang, Xiaowei
Yang, Minlan
Zhang, Fuming
Quan, Chengshi
Li, Yu-Lin
Lu, Shi-Jiang
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-09
Country of Publication
Egypt
No. of Pages
16
Abstract EN
Shortage of red blood cells (RBCs, erythrocytes) can have potentially life-threatening consequences for rare or unusual blood type patients with massive blood loss resulting from various conditions.
Erythrocytes have been derived from human pluripotent stem cells (PSCs), but the risk of potential tumorigenicity cannot be ignored, and a majority of these cells produced from PSCs express embryonic ε- and fetal γ-globins with little or no adult β-globin and remain nucleated.
Here we report a method to generate erythrocytes from human hair follicle mesenchymal stem cells (hHFMSCs) by enforcing OCT4 gene expression and cytokine stimulation.
Cells generated from hHFMSCs expressed mainly the adult β-globin chain with minimum level of the fetal γ-globin chain.
Furthermore, these cells also underwent multiple maturation events and formed enucleated erythrocytes with a biconcave disc shape.
Gene expression analyses showed that OCT4 regulated the expression of genes associated with both pluripotency and erythroid development during hHFMSC transdifferentiation toward erythroid cells.
These findings show that mature erythrocytes can be generated from adult somatic cells, which may serve as an alternative source of RBCs for potential autologous transfusion.
American Psychological Association (APA)
Liu, Zhijing& Lu, Shi-Jiang& Lu, Yan& Tan, Xiaohua& Zhang, Xiaowei& Yang, Minlan…[et al.]. 2015. Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4. Stem Cells International،Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1076199
Modern Language Association (MLA)
Liu, Zhijing…[et al.]. Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4. Stem Cells International No. 2015 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1076199
American Medical Association (AMA)
Liu, Zhijing& Lu, Shi-Jiang& Lu, Yan& Tan, Xiaohua& Zhang, Xiaowei& Yang, Minlan…[et al.]. Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1076199
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076199