WNTβ-Catenin Signaling Is Required for Integration of CD24+ Renal Progenitor Cells into Glycerol-Damaged Adult Renal Tubules
Joint Authors
Zhang, Zhao
Iglesias, Diana M.
Corsini, Rachel
Chu, LeeLee
Goodyer, Paul
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-18
Country of Publication
Egypt
No. of Pages
11
Abstract EN
During development, nephron progenitor cells (NPC) are induced to differentiate by WNT9b signals from the ureteric bud.
Although nephrogenesis ends in the perinatal period, acute kidney injury (AKI) elicits repopulation of damaged nephrons.
Interestingly, embryonic NPC infused into adult mice with AKI are incorporated into regenerating tubules.
Since WNT/β-catenin signaling is crucial for primary nephrogenesis, we reasoned that it might also be needed for the endogenous repair mechanism and for integration of exogenous NPC.
When we examined glycerol-induced AKI in adult mice bearing a β-catenin/TCF reporter transgene, endogenous tubular cells reexpressed the NPC marker, CD24, and showed widespread β-catenin/TCF signaling.
We isolated CD24+ cells from E15 kidneys of mice with the canonical WNT signaling reporter.
40% of cells responded to WNT3a in vitro and when infused into glycerol-injured adult, the cells exhibited β-catenin/TCF reporter activity when integrated into damaged tubules.
When embryonic CD24+ cells were treated with a β-catenin/TCF pathway inhibitor (IWR-1) prior to infusion into glycerol-injured mice, tubular integration of cells was sharply reduced.
Thus, the endogenous canonical β-catenin/TCF pathway is reactivated during recovery from AKI and is required for integration of exogenous embryonic renal progenitor cells into damaged tubules.
These events appear to recapitulate the WNT-dependent inductive process which drives primary nephrogenesis.
American Psychological Association (APA)
Zhang, Zhao& Iglesias, Diana M.& Corsini, Rachel& Chu, LeeLee& Goodyer, Paul. 2015. WNTβ-Catenin Signaling Is Required for Integration of CD24+ Renal Progenitor Cells into Glycerol-Damaged Adult Renal Tubules. Stem Cells International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1076200
Modern Language Association (MLA)
Zhang, Zhao…[et al.]. WNTβ-Catenin Signaling Is Required for Integration of CD24+ Renal Progenitor Cells into Glycerol-Damaged Adult Renal Tubules. Stem Cells International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1076200
American Medical Association (AMA)
Zhang, Zhao& Iglesias, Diana M.& Corsini, Rachel& Chu, LeeLee& Goodyer, Paul. WNTβ-Catenin Signaling Is Required for Integration of CD24+ Renal Progenitor Cells into Glycerol-Damaged Adult Renal Tubules. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1076200
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076200