RhoMRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells
Joint Authors
Zhang, Rui
Wang, Nan
Zhang, Man
Zhang, Li-Nan
Guo, Zhi-Xia
Luo, Xue-Gang
Zhou, Hao
He, Hong-Peng
Zhang, Tong-Cun
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-08
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Mesenchymal stem cells (MSCs) are known to undergo endothelial differentiation in response to treatment with vascular endothelial growth factor (VEGF), but their angiogenic ability is poorly characterized.
In the present study, we aimed to further investigate the role of Rho/MRTF-A in angiogenesis by MSCs and the effect of the Rho/MRTF-A pathway on the expression of integrins α1β1 and α5β1, which are known to mediate physiological and pathological angiogenesis.
Our results showed that increased expression of α1, α5, and β1 was observed during angiogenesis of differentiated MSCs, and the Rho/MRTF-A signaling pathway was demonstrated to be involved in regulating the expression of integrins α1, α5, and β1.
Luciferase reporter assay and ChIP assay determined that MRTF-A could bind to and transactivate the integrin α1 and α5 promoters.
Treatment with the Rho inhibitor C3 transferase, the Rho-associated protein kinase (ROCK) inhibitor Y27632 or with shMRTF-A inhibited both the upregulation of α1, α5, and β1 as well as angiogenesis.
Furthermore, in human umbilical vein endothelial cells (HUVECs), MRTF-A deletion led to marked reductions in cell migration and vessel network formation compared with the control.
These data demonstrate that Rho/MRTF-A signaling is an important mediator that controls integrin gene expression during MSC-mediated angiogenic processes.
American Psychological Association (APA)
Zhang, Rui& Wang, Nan& Zhang, Man& Zhang, Li-Nan& Guo, Zhi-Xia& Luo, Xue-Gang…[et al.]. 2015. RhoMRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells. Stem Cells International،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1076256
Modern Language Association (MLA)
Zhang, Rui…[et al.]. RhoMRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells. Stem Cells International No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1076256
American Medical Association (AMA)
Zhang, Rui& Wang, Nan& Zhang, Man& Zhang, Li-Nan& Guo, Zhi-Xia& Luo, Xue-Gang…[et al.]. RhoMRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1076256
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076256