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Human Adipose Derived Stem Cells Induced Cell Apoptosis and S Phase Arrest in Bladder Tumor
Joint Authors
Yu, Xi
Su, Boxing
Ge, Peng
Wang, Zicheng
Li, Sen
Huang, Bingwei
Gong, Yanqing
Lin, Jian
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-27
Country of Publication
Egypt
No. of Pages
12
Abstract EN
The aim of this study was to determine the effect of human adipose derived stem cells (ADSCs) on the viability and apoptosis of human bladder cancer cells.
EJ and T24 cells were cocultured with ADSCs or cultured with conditioned medium of ADSCs (ADSC-CM), respectively.
The cell counting and colony formation assay showed ADSCs inhibited the proliferation of EJ and T24 cells.
Cell viability assessment revealed that the secretions of ADSCs, in the form of conditioned medium, were able to decrease cancer cell viability.
Wound-healing assay suggested ADSC-CM suppressed migration of T24 and EJ cells.
Moreover, the results of the flow cytometry indicated that ADSC-CM was capable of inducing apoptosis of T24 cells and inducing S phase cell cycle arrest.
Western blot revealed ADSC-CM increased the expression of cleaved caspase-3 and cleaved PARP, indicating that ADSC-CM induced apoptosis in a caspase-dependent way.
PTEN/PI3K/Akt pathway and Bcl-2 family proteins were involved in the mechanism of this reaction.
Our study indicated that ADSCs may provide a promising and practicable manner for bladder tumor therapy.
American Psychological Association (APA)
Yu, Xi& Su, Boxing& Ge, Peng& Wang, Zicheng& Li, Sen& Huang, Bingwei…[et al.]. 2015. Human Adipose Derived Stem Cells Induced Cell Apoptosis and S Phase Arrest in Bladder Tumor. Stem Cells International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1076288
Modern Language Association (MLA)
Yu, Xi…[et al.]. Human Adipose Derived Stem Cells Induced Cell Apoptosis and S Phase Arrest in Bladder Tumor. Stem Cells International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1076288
American Medical Association (AMA)
Yu, Xi& Su, Boxing& Ge, Peng& Wang, Zicheng& Li, Sen& Huang, Bingwei…[et al.]. Human Adipose Derived Stem Cells Induced Cell Apoptosis and S Phase Arrest in Bladder Tumor. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1076288
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076288