Development of a Xeno-Free Substrate for Human Embryonic Stem Cell Growth

Abstract EN

Traditionally, human embryonic stem cells (hESCs) are cultured on inactivated live feeder cells.

For clinical application using hESCs, there is a requirement to minimize the risk of contamination with animal components.

Extracellular matrix (ECM) derived from feeder cells is the most natural way to provide xeno-free substrates for hESC growth.

In this study, we optimized the step-by-step procedure for ECM processing to develop a xeno-free ECM that supports the growth of undifferentiated hESCs.

In addition, this newly developed xeno-free substrate can be stored at 4°C and is ready to use upon request, which serves as an easier way to amplify hESCs for clinical applications.