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Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells
Joint Authors
Geng, Zhe
Li, Ping
Tan, Li
Song, Houyan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-31
Country of Publication
Egypt
No. of Pages
10
Abstract EN
RNA-binding protein TIAR has been suggested to mediate the translational silencing of ARE-containing mRNAs.
To analyze the functions of TIAR, we established RNAi and genetic rescue assays.
We evaluated the expression of neuroectoderm markers Pax6 and nestin, mesoderm markers brachyury and Flk1, and hypoblast and definitive endoderm markers Sox17 and Gata6 during EB differentiation and found that knockdown TIAR expression restrained the differentiation of E14 cells.
We assessed gene expression levels of Flk-1 and VE-cadherin and observed attenuated differentiation of E14 cells into endothelial cells upon downregulation of TIAR gene expression.
As such, we hypothesized an essential role of TIAR related to EB differentiation.
As TIAR inhibits the translation of c-myc, we proposed that downregulation of TIAR results in restrained differentiation of E14 cells, due in part to the function of c-myc.
We found that TIAR inhibited c-myc expression at the translational level in E14 cells; accordingly, a reduction of TIAR expression promoted self-renewal of pluripotent cells and attenuated differentiation.
Additionally, we established that TIAR inhibited TIA-1 expression at the translational level in E14 cells.
Taken together, we have contributed to the understanding of the regulatory relationships between TIAR and both c-myc and TIA-1.
American Psychological Association (APA)
Geng, Zhe& Li, Ping& Tan, Li& Song, Houyan. 2015. Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells. Stem Cells International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1076318
Modern Language Association (MLA)
Geng, Zhe…[et al.]. Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells. Stem Cells International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1076318
American Medical Association (AMA)
Geng, Zhe& Li, Ping& Tan, Li& Song, Houyan. Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1076318
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076318