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Priming Mesenchymal Stem Cells with Endothelial Growth Medium Boosts Stem Cell Therapy for Systemic Arterial Hypertension
Joint Authors
Vinícius da Silva, Marcos
de Oliveira, Lucas Felipe
Almeida, Thalles Ramos
Ribeiro Machado, Marcus Paulo
Cuba, Marilia Beatriz
Alves, Angélica Cristina
Dias da Silva, Valdo José
Rodrigues, Virmondes
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-02
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Systemic arterial hypertension (SAH), a clinical syndrome characterized by persistent elevation of arterial pressure, is often associated with abnormalities such as microvascular rarefaction, defective angiogenesis, and endothelial dysfunction.
Mesenchymal stem cells (MSCs), which normally induce angiogenesis and improve endothelial function, are defective in SAH.
The central aim of this study was to evaluate whether priming of MSCs with endothelial growth medium (EGM-2) increases their therapeutic effects in spontaneously hypertensive rats (SHRs).
Adult female SHRs were administered an intraperitoneal injection of vehicle solution n = 10 , MSCs cultured in conventional medium (DMEM plus 10% FBS, n = 11 ), or MSCs cultured in conventional medium followed by 72 hours in EGM-2 (pMSC, n = 10 ).
Priming of the MSCs reduced the basal cell death rate in vitro.
The administration of pMSCs significantly induced a prolonged reduction (10 days) in arterial pressure, a decrease in cardiac hypertrophy, an improvement in endothelium-dependent vasodilation response to acetylcholine, and an increase in skeletal muscle microvascular density compared to the vehicle and MSC groups.
The transplanted cells were rarely found in the hearts and kidneys.
Taken together, our findings indicate that priming of MSCs boosts stem cell therapy for the treatment of SAH.
American Psychological Association (APA)
de Oliveira, Lucas Felipe& Almeida, Thalles Ramos& Ribeiro Machado, Marcus Paulo& Cuba, Marilia Beatriz& Alves, Angélica Cristina& Vinícius da Silva, Marcos…[et al.]. 2015. Priming Mesenchymal Stem Cells with Endothelial Growth Medium Boosts Stem Cell Therapy for Systemic Arterial Hypertension. Stem Cells International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1076330
Modern Language Association (MLA)
de Oliveira, Lucas Felipe…[et al.]. Priming Mesenchymal Stem Cells with Endothelial Growth Medium Boosts Stem Cell Therapy for Systemic Arterial Hypertension. Stem Cells International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1076330
American Medical Association (AMA)
de Oliveira, Lucas Felipe& Almeida, Thalles Ramos& Ribeiro Machado, Marcus Paulo& Cuba, Marilia Beatriz& Alves, Angélica Cristina& Vinícius da Silva, Marcos…[et al.]. Priming Mesenchymal Stem Cells with Endothelial Growth Medium Boosts Stem Cell Therapy for Systemic Arterial Hypertension. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1076330
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076330