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Study of Collagen Birefringence in Different Grades of Oral Squamous Cell Carcinoma Using Picrosirius Red and Polarized Light Microscopy
Joint Authors
Arun Gopinathan, Pillai
Kokila, Ganganna
Jyothi, Mahadesh
Ananjan, Chatterjee
Pradeep, Linganna
Humaira Nazir, Salroo
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-26
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objectives.
The present study was done to evaluate birefringence pattern of collagen fibres in different grades of oral squamous cell carcinoma using Picrosirius red stain and polarization microscopy and to determine if there is a change in collagen fibres between different grades of oral squamous cell carcinoma.
Materials and Methods.
Picrosirius red stained 5 μm thick sections of previously diagnosed different grades of squamous cell carcinoma and normal oral mucosa were studied under polarization microscopy for arrangement as well as birefringence of collagen fibres around tumour islands.
Results.
It was found that thin collagen fibres increased and thick collagen fibres decreased with dedifferentiation of OSCC (P<0.0001 ).
It was observed that there was change in polarization colours of thick fibres from yellowish orange to greenish yellow with dedifferentiation of OSCC indicating loosely packed fibres (P<0.0001).
Conclusion.
There was a gradual change of birefringence of collagen from yellowish orange to greenish yellow from well to poorly differentiated squamous cell carcinoma, indicating that there is a change from mature form of collagen to immature form as tumour progresses.
Studying collagen fibres with Picrosirius red for stromal changes around tumour islands along with routine staining may help in predicting the prognosis of tumour.
American Psychological Association (APA)
Arun Gopinathan, Pillai& Kokila, Ganganna& Jyothi, Mahadesh& Ananjan, Chatterjee& Pradeep, Linganna& Humaira Nazir, Salroo. 2015. Study of Collagen Birefringence in Different Grades of Oral Squamous Cell Carcinoma Using Picrosirius Red and Polarized Light Microscopy. Scientifica،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1076462
Modern Language Association (MLA)
Arun Gopinathan, Pillai…[et al.]. Study of Collagen Birefringence in Different Grades of Oral Squamous Cell Carcinoma Using Picrosirius Red and Polarized Light Microscopy. Scientifica No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1076462
American Medical Association (AMA)
Arun Gopinathan, Pillai& Kokila, Ganganna& Jyothi, Mahadesh& Ananjan, Chatterjee& Pradeep, Linganna& Humaira Nazir, Salroo. Study of Collagen Birefringence in Different Grades of Oral Squamous Cell Carcinoma Using Picrosirius Red and Polarized Light Microscopy. Scientifica. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1076462
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076462