An Improved LC-MSMS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations
Joint Authors
Chang, Yanxu
Gao, Xiu-Mei
Li, Jin
Zhang, Qiu-Hong
He, Jun
Liu, Erwei
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
An improved LC-MS/MS method was developed for simultaneous determination of eleven bioactive constituents of Radix Angelicae Pubescentis and its related preparations.
It was the first report on the quantification of bioactive constituents in different preparations of Radix Angelicae Pubescentis by LC-MS/MS analytical method.
These samples were separated with an Agilent Zorbax Extend reversed-phase C18 column (1.8 μm, 4.6 × 100 mm) by linear gradient elution using aqueous ammonium acetate and acetonitrile as mobile phase.
The flow rate was 0.3 mL min−1.
The eleven bioactive constituents showed good regression (R>0.990) within test ranges and the recoveries were in the range of 87.1–110%.
The limit of detections and quantifications for most of the major constituents were less than 0.5 and 1.0 ng mL−1, respectively.
All results indicated that the developed method could be readily utilized as a suitable quality control method for Radix Angelicae Pubescentis and related preparations.
American Psychological Association (APA)
Li, Jin& Zhang, Qiu-Hong& He, Jun& Liu, Erwei& Gao, Xiu-Mei& Chang, Yanxu. 2015. An Improved LC-MSMS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations. The Scientific World Journal،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1078704
Modern Language Association (MLA)
Li, Jin…[et al.]. An Improved LC-MSMS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations. The Scientific World Journal No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1078704
American Medical Association (AMA)
Li, Jin& Zhang, Qiu-Hong& He, Jun& Liu, Erwei& Gao, Xiu-Mei& Chang, Yanxu. An Improved LC-MSMS Method for Simultaneous Determination of the Eleven Bioactive Constituents for Quality Control of Radix Angelicae Pubescentis and Its Related Preparations. The Scientific World Journal. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1078704
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1078704