Decolorization of Anthraquinonic Dyes from Textile Effluent Using Horseradish Peroxidase: Optimization and Kinetic Study
Joint Authors
Knežević-Jugović, Zorica
Šekuljica, Nataša Ž.
Prlainović, Nevena Ž.
Stefanović, Andrea B.
Žuža, Milena G.
Čičkarić, Dragana Z.
Mijin, Dušan Ž.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Two anthraquinonic dyes, C.I.
Acid Blue 225 and C.I.
Acid Violet 109, were used as models to explore the feasibility of using the horseradish peroxidase enzyme (HRP) in the practical decolorization of anthraquinonic dyes in wastewater.
The influence of process parameters such as enzyme concentration, hydrogen peroxide concentration, temperature, dye concentration, and pH was examined.
The pH and temperature activity profiles were similar for decolorization of both dyes.
Under the optimal conditions, 94.7% of C.I.
Acid Violet 109 from aqueous solution was decolorized (treatment time 15 min, enzyme concentration 0.15 IU/mL, hydrogen peroxide concentration 0.4 mM, dye concentration 30 mg/L, pH 4, and temperature 24°C) and 89.36% of C.I.
Acid Blue 225 (32 min, enzyme concentration 0.15 IU/mL, hydrogen peroxide concentration 0.04 mM, dye concentration 30 mg/L, pH 5, and temperature 24°C).
The mechanism of both reactions has been proven to follow the two substrate ping-pong mechanism with substrate inhibition, revealing the formation of a nonproductive or dead-end complex between dye and HRP or between H2O2 and the oxidized form of the enzyme.
Both chemical oxygen demand and total organic carbon values showed that there was a reduction in toxicity after the enzymatic treatment.
This study verifies the viability of use of horseradish peroxidase for the wastewaters treatment of similar anthraquinonic dyes.
American Psychological Association (APA)
Šekuljica, Nataša Ž.& Prlainović, Nevena Ž.& Stefanović, Andrea B.& Žuža, Milena G.& Čičkarić, Dragana Z.& Mijin, Dušan Ž.…[et al.]. 2015. Decolorization of Anthraquinonic Dyes from Textile Effluent Using Horseradish Peroxidase: Optimization and Kinetic Study. The Scientific World Journal،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1078710
Modern Language Association (MLA)
Šekuljica, Nataša Ž.…[et al.]. Decolorization of Anthraquinonic Dyes from Textile Effluent Using Horseradish Peroxidase: Optimization and Kinetic Study. The Scientific World Journal No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1078710
American Medical Association (AMA)
Šekuljica, Nataša Ž.& Prlainović, Nevena Ž.& Stefanović, Andrea B.& Žuža, Milena G.& Čičkarić, Dragana Z.& Mijin, Dušan Ž.…[et al.]. Decolorization of Anthraquinonic Dyes from Textile Effluent Using Horseradish Peroxidase: Optimization and Kinetic Study. The Scientific World Journal. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1078710
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1078710