Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine
Joint Authors
Shimojo, Andréa Arruda Martins
Perez, Amanda Gomes Marcelino
Galdames, Sofia Elisa Moraga
Brissac, Isabela Cambraia de Souza
Santana, Maria Helena A.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-02
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
The sponges were prepared by controlled freezing (−20, −80, or −196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v).
The platelet-rich plasma was obtained from controlled centrifugation of whole blood and activated with calcium and autologous serum.
The composite scaffolds were prepared by embedding the sponges with the activated platelet-rich plasma.
The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells.
The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a −20°C freezing temperature, while −196°C favored osteogenic differentiation.
Although the composite scaffolds are promising for regenerative medicine, the structures require stabilization to prevent the collapse observed after five days.
American Psychological Association (APA)
Shimojo, Andréa Arruda Martins& Perez, Amanda Gomes Marcelino& Galdames, Sofia Elisa Moraga& Brissac, Isabela Cambraia de Souza& Santana, Maria Helena A.. 2015. Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine. The Scientific World Journal،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1078731
Modern Language Association (MLA)
Shimojo, Andréa Arruda Martins…[et al.]. Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine. The Scientific World Journal No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1078731
American Medical Association (AMA)
Shimojo, Andréa Arruda Martins& Perez, Amanda Gomes Marcelino& Galdames, Sofia Elisa Moraga& Brissac, Isabela Cambraia de Souza& Santana, Maria Helena A.. Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine. The Scientific World Journal. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1078731
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1078731