The Effects of Tempol on Cyclophosphamide-Induced Oxidative Stress in Rat Micturition Reflexes
Joint Authors
Vizzard, Margaret A.
Peterson, Abbey
Malley, Susan
Daniel, Mitchel
Lambert, Daniel
Kosofsky, Michael
Gonzalez, Eric J.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
We hypothesized that cyclophosphamide- (CYP-) induced cystitis results in oxidative stress and contributes to urinary bladder dysfunction.
We determined (1) the expression of oxidative stress markers 3-nitrotyrosine (3-NT), reactive oxygen species (ROS)/reactive nitrogen species (RNS), inflammatory modulators, neuropeptides calcitonin gene-related peptide (CGRP), substance P (Sub P), and adenosine triphosphate (ATP) that contribute to the inflammatory process in the urinary tract and (2) the functional role of oxidative stress in urinary bladder dysfunction with an antioxidant, Tempol, (1 mM in drinking water) combined with conscious cystometry.
In CYP-treated (4 hr or 48 hr; 150 mg/kg, i.p.) rats, ROS/RNS and 3-NT significantly (P≤0.01) increased in urinary bladder.
CYP treatment increased ATP, Sub P, and CGRP expression in the urinary bladder and cystometric fluid.
In CYP-treated rats, Tempol significantly (P≤0.01) increased bladder capacity and reduced voiding frequency compared to CYP-treated rats without Tempol.
Tempol significantly (P≤0.01) reduced ATP expression, 3-NT, and ROS/RNS expression in the urinary tract of CYP-treated rats.
These studies demonstrate that reducing oxidative stress in CYP-induced cystitis improves urinary bladder function and reduces markers of oxidative stress and inflammation.
American Psychological Association (APA)
Gonzalez, Eric J.& Peterson, Abbey& Malley, Susan& Daniel, Mitchel& Lambert, Daniel& Kosofsky, Michael…[et al.]. 2015. The Effects of Tempol on Cyclophosphamide-Induced Oxidative Stress in Rat Micturition Reflexes. The Scientific World Journal،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1078882
Modern Language Association (MLA)
Gonzalez, Eric J.…[et al.]. The Effects of Tempol on Cyclophosphamide-Induced Oxidative Stress in Rat Micturition Reflexes. The Scientific World Journal No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1078882
American Medical Association (AMA)
Gonzalez, Eric J.& Peterson, Abbey& Malley, Susan& Daniel, Mitchel& Lambert, Daniel& Kosofsky, Michael…[et al.]. The Effects of Tempol on Cyclophosphamide-Induced Oxidative Stress in Rat Micturition Reflexes. The Scientific World Journal. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1078882
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1078882