Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics
Joint Authors
Brayner, Fábio André
Feitosa, Ana Paula Sampaio
Alves, Luiz Carlos
Veras, Dyana Leal
de Souza Lopes, Ana Catarina
Vaz da Silva, Grasielle
Araújo Gonçalves, Gabriel Gazzoni
de Freitas, Catarina Fernandes
de Lima, Fernanda Cristina Gomes
Vieira Maciel, Maria Amélia
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-28
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The aim of this study was to characterize the ultrastructural effects caused by β-lactam antibiotics in Klebsiella pneumoniae isolates.
Three K.
pneumoniae clinical isolates were selected for the study with resistance profiles for third-generation cephalosporins, aztreonam, and/or imipenem and with different resistance genes for extended-spectrum β-lactamases (ESBL) or Klebsiella pneumoniae carbapenemase (KPC).
Two K.
pneumoniae isolates obtained from the microbiota, which were both resistant to amoxicillin and ampicillin, were also analyzed.
In accordance with the susceptibility profile, the clinical isolates were subjected to subminimum inhibitory concentrations (sub-MICs) of cefotaxime, ceftazidime, aztreonam, and imipenem and the isolates from the microbiota to ampicillin and amoxicillin, for analysis by means of scanning and transmission electron microscopy.
The K.
pneumoniae isolates showed different morphological and ultrastructural changes after subjection to β-lactams tested at different concentrations, such as cell filamentation, loss of cytoplasmic material, and deformation of dividing septa.
Our results demonstrate that K.
pneumoniae isolates harboring different genes that encode for β-lactamases show cell alterations when subjected to different β-lactam antibiotics, thus suggesting that they possess residual activity in vitro, despite the phenotypic resistance presented in the isolates analyzed.
American Psychological Association (APA)
Veras, Dyana Leal& de Souza Lopes, Ana Catarina& Vaz da Silva, Grasielle& Araújo Gonçalves, Gabriel Gazzoni& de Freitas, Catarina Fernandes& de Lima, Fernanda Cristina Gomes…[et al.]. 2015. Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics. The Scientific World Journal،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1078895
Modern Language Association (MLA)
Veras, Dyana Leal…[et al.]. Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics. The Scientific World Journal No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1078895
American Medical Association (AMA)
Veras, Dyana Leal& de Souza Lopes, Ana Catarina& Vaz da Silva, Grasielle& Araújo Gonçalves, Gabriel Gazzoni& de Freitas, Catarina Fernandes& de Lima, Fernanda Cristina Gomes…[et al.]. Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics. The Scientific World Journal. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1078895
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1078895