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Laser Phototherapy Enhances Mesenchymal Stem Cells Survival in Response to the Dental Adhesives
Joint Authors
Diniz, Ivana Márcia Alves
Matos, Adriana Bona
Marques, Márcia Martins
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-23
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Background.
We investigated the influence of laser phototherapy (LPT) on the survival of human mesenchymal stem cells (MSCs) submitted to substances leached from dental adhesives.
Method.
MSCs were isolated and characterized.
Oral mucosa fibroblasts and osteoblast-like cells were used as comparative controls.
Cultured medium conditioned with two adhesive systems was applied to the cultures.
Cell monolayers were exposed or not to LPT.
Laser irradiations were performed using a red laser (GaAlAs, 780 nm, 0.04 cm2, 40 mW, 1 W/cm2, 0.4 J, 10 seconds, 1 point, 10 J/cm2).
After 24 h, cell viability was assessed by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide reduction assay.
Data were statistically compared by ANOVA followed by Tukey’s test (P<0.05).
Results.
Different cell types showed different viabilities in response to the same materials.
Substances leached from adhesives were less cytotoxic to MSCs than to other cell types.
Substances leached from Clearfil SE Bond were highly cytotoxic to all cell types tested, except to the MSCs when applied polymerized and in association with LPT.
LPT was unable to significantly increase the cell viability of fibroblasts and osteoblast-like cells submitted to the dental adhesives.
Conclusion.
LPT enhances mesenchymal stem cells survival in response to substances leached from dental adhesives.
American Psychological Association (APA)
Diniz, Ivana Márcia Alves& Matos, Adriana Bona& Marques, Márcia Martins. 2015. Laser Phototherapy Enhances Mesenchymal Stem Cells Survival in Response to the Dental Adhesives. The Scientific World Journal،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1078970
Modern Language Association (MLA)
Diniz, Ivana Márcia Alves…[et al.]. Laser Phototherapy Enhances Mesenchymal Stem Cells Survival in Response to the Dental Adhesives. The Scientific World Journal No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1078970
American Medical Association (AMA)
Diniz, Ivana Márcia Alves& Matos, Adriana Bona& Marques, Márcia Martins. Laser Phototherapy Enhances Mesenchymal Stem Cells Survival in Response to the Dental Adhesives. The Scientific World Journal. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1078970
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1078970