Effects of TGF-β1 on OPGRANKL Expression of Cementoblasts and Osteoblasts Are Similar without Stress but Different with Mechanical Compressive Stress

Abstract EN

Introduction.

This study aimed to explore the effects of TGF-β1 on regulating activities of cementoblasts and osteoblasts with or without stress.

Material and Methods.

Human recombinant TGF-β1 was added with different doses.

Immunohistochemical test of osteoprotegerin (OPG)/receptor activator of nuclear factor-kappaB ligand (RANKL) and Alizarin Red-S staining were conducted.

Mechanical compressive stress was obtained by increasing the pressure of gaseous phase.

OPG/RANKL expression was detected in both cells through quantitative real-time PCR.

Results.

Similar significant differences ( P < 0.05 ) existed in OPG/RANKL change with increasing concentration of TGF-β1 without mechanical stress for cementoblasts and osteoblasts.

However, under 3 h stress, OPG increased and RANKL decreased significantly ( P < 0.01 ) but with similar OPG/RANKL change.

Moreover, under 24 h stress, OPG change exhibited no difference ( P > 0.05 ), but RANKL decreased significantly ( P < 0.01 ) at 10 and 100 ng/mL TGF-β1 in cementoblasts.

In osteoblasts, OPG increased significantly ( P < 0.01 ) at 10 and 100 ng/mL, whereas RANKL decreased with statistical difference ( P < 0.05 ) at 1 and 10 ng/mL.

Conclusions.

The effects of TGF-β1 on OPG/RANKL expression of cementoblasts and osteoblasts are similar even without mechanical stress.

However, these effects are different under mechanical compressive stress.