Qualitative and Quantitative Analysis of the Major Constituents in Chinese Medical Preparation Lianhua-Qingwen Capsule by UPLC-DAD-QTOF-MS
Joint Authors
Zhu, Yan
Jia, Weina
Wang, Chunhua
Wang, Yuefei
Pan, Guixiang
Jiang, Miaomiao
Li, Zheng
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-08
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Lianhua-Qingwen capsule (LQC) is a commonly used Chinese medical preparation to treat viral influenza and especially played a very important role in the fight against severe acute respiratory syndrome (SARS) in 2002-2003 in China.
In this paper, a rapid ultraperformance liquid chromatography coupled with diode-array detector and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF-MS) method was established for qualitative and quantitative analysis of the major constituents of LQC.
A total of 61 compounds including flavonoids, phenylpropanoids, anthraquinones, triterpenoids, iridoids, and other types of compounds were unambiguously or tentatively identified by comparing the retention times and accurate mass measurement with reference compounds or literature data.
Among them, twelve representative compounds were further quantified as chemical markers in quantitative analysis, including salidroside, chlorogenic acid, forsythoside E, cryptochlorogenic acid, amygdalin, sweroside, hyperin, rutin, forsythoside A, phillyrin, rhein, and glycyrrhizic acid.
The UPLC-DAD method was evaluated with linearity, limit of detection (LOD), limit of quantification (LOQ), precision, stability, repeatability, and recovery tests.
The results showed that the developed quantitative method was linear, sensitive, and precise for the quality control of LQC.
American Psychological Association (APA)
Jia, Weina& Wang, Chunhua& Wang, Yuefei& Pan, Guixiang& Jiang, Miaomiao& Li, Zheng…[et al.]. 2015. Qualitative and Quantitative Analysis of the Major Constituents in Chinese Medical Preparation Lianhua-Qingwen Capsule by UPLC-DAD-QTOF-MS. The Scientific World Journal،Vol. 2015, no. 2015, pp.1-19.
https://search.emarefa.net/detail/BIM-1079068
Modern Language Association (MLA)
Jia, Weina…[et al.]. Qualitative and Quantitative Analysis of the Major Constituents in Chinese Medical Preparation Lianhua-Qingwen Capsule by UPLC-DAD-QTOF-MS. The Scientific World Journal No. 2015 (2015), pp.1-19.
https://search.emarefa.net/detail/BIM-1079068
American Medical Association (AMA)
Jia, Weina& Wang, Chunhua& Wang, Yuefei& Pan, Guixiang& Jiang, Miaomiao& Li, Zheng…[et al.]. Qualitative and Quantitative Analysis of the Major Constituents in Chinese Medical Preparation Lianhua-Qingwen Capsule by UPLC-DAD-QTOF-MS. The Scientific World Journal. 2015. Vol. 2015, no. 2015, pp.1-19.
https://search.emarefa.net/detail/BIM-1079068
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1079068