Inhibition of Pasteurella multocida Adhesion to Rabbit Respiratory Epithelium Using Lectins
Joint Authors
Carrillo, Magda Patricia
Martinez, Nhora María
Patiño, María del Pilar
Iregui, Carlos
Source
Veterinary Medicine International
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
This study aimed to evaluate the ability of a panel of lectins to inhibit the ability of Pasteurella multocida to adhere to and affect the rabbit respiratory epithelium.
Nasal septa from rabbit fetuses were cultured with various lectins before the addition of P.
multocida.
The percentage of bacteria adhering to the epithelium was evaluated semiquantitatively by indirect immunoperoxidase (IIP) staining.
The goblet cells (GCs) were counted in semithin sections stained with toluidine blue and served as the main morphological criterion to evaluate the inhibitory effect of the lectins.
The lectins PNA, WGA, RCA120, and DBA significantly inhibited the adhesion of P.
multocida to the ciliated epithelium P<0.05 and prevented the pathogen-induced increase in the number of GCs P<0.05 compared with those of positive control tissues.
In addition, VVA, SJA, UEA I, DSL, SBA, and ECL significantly inhibited the increase in GCs compared with that of the control tissues.
The results suggest that less aggressive therapeutic strategies, such as treatment with lectins, may represent alternative approaches to control bacterial respiratory infections.
American Psychological Association (APA)
Carrillo, Magda Patricia& Martinez, Nhora María& Patiño, María del Pilar& Iregui, Carlos. 2015. Inhibition of Pasteurella multocida Adhesion to Rabbit Respiratory Epithelium Using Lectins. Veterinary Medicine International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1079381
Modern Language Association (MLA)
Carrillo, Magda Patricia…[et al.]. Inhibition of Pasteurella multocida Adhesion to Rabbit Respiratory Epithelium Using Lectins. Veterinary Medicine International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1079381
American Medical Association (AMA)
Carrillo, Magda Patricia& Martinez, Nhora María& Patiño, María del Pilar& Iregui, Carlos. Inhibition of Pasteurella multocida Adhesion to Rabbit Respiratory Epithelium Using Lectins. Veterinary Medicine International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1079381
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1079381