Platelet-Rich Gel Supernatants Stimulate the Release of Anti-Inflammatory Proteins on Culture Media of Normal Equine Synovial Membrane Explants
Joint Authors
Carmona, J. U.
López, Catalina
Ríos, Diana L.
Source
Veterinary Medicine International
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The aims were as follows: (1) to evaluate the effects at 48 and 96 h of two concentrations (25 and 50%) of leukocyte and platelet-rich gel (L-PRG) and pure PRG (P-PRG) supernatants on the production/degradation in normal equine synovial membrane explants (SME) of platelet derived growth factor isoform BB, transforming growth factor beta-1, tumor necrosis factor alpha, interleukin (IL-) 4 (IL-4), IL-1 receptor antagonist (IL-1ra), and hyaluronan (HA) synthesis and (2) to correlate these molecules with their respective PRG supernatant treatments.
SME from 6 horses were cultured for 96 h with L-PRG and P-PRG supernatants at 25 and 50% concentrations, respectively.
SME culture media were changed each 48 h and used for determination by ELISA of the molecules, which were also determined in synovial fluid.
25% L-PRG supernatant produced a sustained release over time of IL-1ra and a gradual release of HA, whereas 50% L-PRG supernatant produced a sustained increase over time of IL-4 and HA.
50% P-PRG supernatant produced an increased and sustained production of IL-1ra and IL-4.
The cellular composition and the articular concentration (volume) of a platelet-rich plasma preparation could affect the anti-inflammatory and anabolic joint responses in horses with osteoarthritis.
American Psychological Association (APA)
Ríos, Diana L.& López, Catalina& Carmona, J. U.. 2015. Platelet-Rich Gel Supernatants Stimulate the Release of Anti-Inflammatory Proteins on Culture Media of Normal Equine Synovial Membrane Explants. Veterinary Medicine International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1079383
Modern Language Association (MLA)
Ríos, Diana L.…[et al.]. Platelet-Rich Gel Supernatants Stimulate the Release of Anti-Inflammatory Proteins on Culture Media of Normal Equine Synovial Membrane Explants. Veterinary Medicine International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1079383
American Medical Association (AMA)
Ríos, Diana L.& López, Catalina& Carmona, J. U.. Platelet-Rich Gel Supernatants Stimulate the Release of Anti-Inflammatory Proteins on Culture Media of Normal Equine Synovial Membrane Explants. Veterinary Medicine International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1079383
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1079383