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Isolation and identification of antibiotic producing pseudomonas fluorescens NBRC-14160 from Delta soil in Egypt
Other Title(s)
عزل و تعريف بكتيريا السيدوموناس فلوروسنس NBRCI4I6O المنتجة للمضادات الحيوية من أرض الدلتا بمصر
Joint Authors
Radwan, Wafa Hamdi
Abu Husayn, Samah Hashim
Salim, Shawqi Mahmud
Yahya, Mahmud Samih
Muhammad, Muhammad Karam al-Din
Uthman, Mustafa Sabri
Mustafa, Dua Nasr
Jumah, Muhammad Muhammad
Fahmi, Muhammad Ahmad
Shaban, Rumaysa Hatim
Kamil, Ahmad Mustafa
Abd al-Hayy, Mustafa Fathi
Source
Arab Universities Journal of Agricultural Sciences
Issue
Vol. 28, Issue 3 (30 Sep. 2020), pp.797-808, 12 p.
Publisher
Ain Shams University Faculty of Agriculture
Publication Date
2020-09-30
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Topics
- Activities
- Biological control
- Chemical reagents
- Antimicrobials
- Pathogenic bacteria
- Staphylococcus
- Aspergillus niger
Abstract EN
Pseudomonas fluorescens is one of plant Growth Promoting Rhizobacteria (PGPR) members which has a major role in the biological control of bacterial and fungal pathogens.
A research was conducted at the Laboratory of Microbiology, Faculty of Agriculture, Ain Shams University, Qalubyia governorate intended for isolating Pseudomonas fluorescens isolate, efficient in antibiotic production.
For isolation, soil samples, collected from Faculty of agriculture farm soil at depth of 10 cm, were screened for Ps.
fluorescens isolates by cultivating the samples on King's medium.
Out of 30 isolates obtained, one was selected based on its high lipid content, because of the lipid's correlation to antibiotic production and inhibitory activity.
The selected isolate was characterized by morphological, physiological, biochemical tests then confirmed its identity by 16S rRNA gene sequence analysis and named Pseudomonas fluorescens NBRC-14160 16srRNA.
Morphological features showed that Ps.
fluorescens NBRC-14160 has large colonies, with irregular surface, opaque, producing green fluorescent pigments.
Cells are short rods, Gram negative.
Physiological features indicated that Ps.
fluorescens NBRC-14160 is capable of producing several exoenzymes including lipase, phospholipase, protease and chitinase and incapable of producing amylase and cellulase.
Carbohydrate fermentation tests were positive for fructose, glucose, D-glucose, and galactose, forming acids after 24 hrs.
of incubation at 30˚C.
However, it loses the ability to ferment inositol, mannose, xylose, mannitol, raffinose and rhamnose sugars.
It's lipid content was 607 mg/g.
Inhibitory activity was studied by the method of disc diffusion test against nine pathogenic bacterial and fungal strains.
Staphylococcus aureus was the most sensitive bacterial pathogen towards P.
fluorescens NBRC-14160 with a 4.5 cm zone of inhibition, while Serratia marscens had 0.95 cm zone.
Aspergillus niger and Alternaria solani were the most sensitive fungal pathogens towards P.
fluorescens NBRC-14160 with 2.55 and 2.5 cm of inhibition zone, respectively.
However, the most resistant fungus was Fusarium oxysporum with 1.3 cm inhibition zone.
American Psychological Association (APA)
Yahya, Mahmud Samih& Muhammad, Muhammad Karam al-Din& Uthman, Mustafa Sabri& Mustafa, Dua Nasr& Jumah, Muhammad Muhammad& Fahmi, Muhammad Ahmad…[et al.]. 2020. Isolation and identification of antibiotic producing pseudomonas fluorescens NBRC-14160 from Delta soil in Egypt. Arab Universities Journal of Agricultural Sciences،Vol. 28, no. 3, pp.797-808.
https://search.emarefa.net/detail/BIM-1079779
Modern Language Association (MLA)
Yahya, Mahmud Samih…[et al.]. Isolation and identification of antibiotic producing pseudomonas fluorescens NBRC-14160 from Delta soil in Egypt. Arab Universities Journal of Agricultural Sciences Vol. 28, no. 3 (2020), pp.797-808.
https://search.emarefa.net/detail/BIM-1079779
American Medical Association (AMA)
Yahya, Mahmud Samih& Muhammad, Muhammad Karam al-Din& Uthman, Mustafa Sabri& Mustafa, Dua Nasr& Jumah, Muhammad Muhammad& Fahmi, Muhammad Ahmad…[et al.]. Isolation and identification of antibiotic producing pseudomonas fluorescens NBRC-14160 from Delta soil in Egypt. Arab Universities Journal of Agricultural Sciences. 2020. Vol. 28, no. 3, pp.797-808.
https://search.emarefa.net/detail/BIM-1079779
Data Type
Journal Articles
Language
English
Notes
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Record ID
BIM-1079779