Detection of the timing of human skin wounds by immunohistochemical analysis of CD14
Other Title(s)
تحديد وقت حدوث الجروح الجلدية عند الإنسان بواسطة الصبغة المناعية النسيجية الكيميائية سى دي 14
Joint Authors
Fuad, Azzah A.
Badr al-Din, Fatimah M. M.
Manisi, Hibah M. K. al-Din
Abd al-Latif, Amani A.
Khidr, Rasha I.
Source
Arab Journal of Forensic Sciences and Forensic Medicine
Issue
Vol. 1, Issue 10 (31 Dec. 2019), pp.1346-1357, 12 p.
Publisher
Naif Arab University for Security Sciences Arab Society for Forensic Sciences and Forensic Medicine
Publication Date
2019-12-31
Country of Publication
Saudi Arabia
No. of Pages
12
Main Subjects
Abstract EN
Determination of time of injury is one of the most important topics in forensic autopsy.
Several methods have been developed to estimate wound age, but unfortunately with limited success.
The aim of the present work was to evaluate the efficacy of Cluster of Differentiation 14 (CD14) as a reliable marker for estimating wound age.
The study was conducted on forty victims with different types of wound and known infliction time.
Skin samples were obtained during autopsy from the center of the wound.
Sections from samples were histologically examined by H & E stain.
Immunohistochemical staining was done using CD14 antibody and the staining density was evaluated semi-quantitatively.
There was a statistically significant relation between wound age and percentage of CD14 expression.
Expression of CD14 was 61.81±6.55 % in specimens from wounds aged less than 12 hours.
It increased till reaching its maximum (96.40±3.78 %) for wounds aged between 1-3 days.
Then it decreased dramatically to 14.80±3.49 % in wounds older than 3 days.
CD14 is proved to be a reliable marker for estimating wound age.
It gave best results in wounds aged between 1-3 days with an overall accuracy of 100%.
Accordingly, it can be used to determine wound age in medicolegal practice.
American Psychological Association (APA)
Fuad, Azzah A.& Badr al-Din, Fatimah M. M.& Manisi, Hibah M. K. al-Din& Abd al-Latif, Amani A.& Khidr, Rasha I.. 2019. Detection of the timing of human skin wounds by immunohistochemical analysis of CD14. Arab Journal of Forensic Sciences and Forensic Medicine،Vol. 1, no. 10, pp.1346-1357.
https://search.emarefa.net/detail/BIM-1086536
Modern Language Association (MLA)
Fuad, Azzah A.…[et al.]. Detection of the timing of human skin wounds by immunohistochemical analysis of CD14. Arab Journal of Forensic Sciences and Forensic Medicine Vol. 1, no. 10 (Dec. 2019), pp.1346-1357.
https://search.emarefa.net/detail/BIM-1086536
American Medical Association (AMA)
Fuad, Azzah A.& Badr al-Din, Fatimah M. M.& Manisi, Hibah M. K. al-Din& Abd al-Latif, Amani A.& Khidr, Rasha I.. Detection of the timing of human skin wounds by immunohistochemical analysis of CD14. Arab Journal of Forensic Sciences and Forensic Medicine. 2019. Vol. 1, no. 10, pp.1346-1357.
https://search.emarefa.net/detail/BIM-1086536
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-1086536