Correlation between biofilm formation, multi-drug resistance and algd gene among pseudomonas aeruginosa clinical isolates
Joint Authors
Namuq, Asma O.
Ali, Khalid Muhammad O.
Al-Ani, Ahmad Hasan
Source
Journal of Babylon University : Journal of Applied and Pure Sciences
Issue
Vol. 27, Issue 3 (30 Jun. 2019), pp.143-150, 8 p.
Publisher
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Abstract EN
Background: Pseudomonas aeruginosa is an opportunistic pathogenic bacterium that associated with persistent infections that are difficult to eradicate.
Objective: This study aims to determine biofilm forming potential of P.
aeruginosa and its correlation with antibiotics resistance and presence of AlgD gene.
Materilals and Methods: The present study carried out in Azady teaching general hospital in Kirkuk city during the period from August 2017 to May 2018, one hundred isolates of Pseudomonas aeruginosa had been obtained from (1260) different clinical specimens.
Antibiotic susceptibility was determined by Kirby- Bauer method, biofilm formation analyzed by Microtiter plate quantitative method, and AlgD gene was determined by polymerase chain reaction (PCR).Results: A lower percentage of antibiotic resistance was against piperacillin/tazobactam (5%), cefepime (24%), and ceftazidime (26%) while, higher resistance percentage was seen against amoxicillin/clavulanic acid (98%) and trimethoprim/sulfamethoxazole (97%), multi-drug resistance (MDR) formed (60%) of total isolates.
Biofilm formation found in (98%) of total isolates.
AlgD gene detected by PCR technique found in (98%) of total isolates, AlgD gene was found only in biofilm former isolates.
Conclusions: Significant correlation found between biofilm formation and antibiotic resistance.
Strong correlation observed between presence of AlgD gene and biofilm formation.
Key Words: Multi-drug resistance (MDR), biofilm formation, AlgD gene.
American Psychological Association (APA)
Namuq, Asma O.& Ali, Khalid Muhammad O.& Al-Ani, Ahmad Hasan. 2019. Correlation between biofilm formation, multi-drug resistance and algd gene among pseudomonas aeruginosa clinical isolates. Journal of Babylon University : Journal of Applied and Pure Sciences،Vol. 27, no. 3, pp.143-150.
https://search.emarefa.net/detail/BIM-1088036
Modern Language Association (MLA)
Namuq, Asma O.…[et al.]. Correlation between biofilm formation, multi-drug resistance and algd gene among pseudomonas aeruginosa clinical isolates. Journal of Babylon University : Journal of Applied and Pure Sciences Vol. 27, no. 3 (2019), pp.143-150.
https://search.emarefa.net/detail/BIM-1088036
American Medical Association (AMA)
Namuq, Asma O.& Ali, Khalid Muhammad O.& Al-Ani, Ahmad Hasan. Correlation between biofilm formation, multi-drug resistance and algd gene among pseudomonas aeruginosa clinical isolates. Journal of Babylon University : Journal of Applied and Pure Sciences. 2019. Vol. 27, no. 3, pp.143-150.
https://search.emarefa.net/detail/BIM-1088036
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 148-149
Record ID
BIM-1088036