Micropropagation of black nightshade (Solanum nigrum L.) : a promising medicinal plant in Libya
Other Title(s)
الإكثار الدقيق لنبات عنب الذئب (Solanum nigrum L.) : نبات طبي واعد ينمو في البرية في ليبيا
Joint Authors
Yunus, Layla Sasi
Tahtamuni, Riham Walid
al-Qudah, Tamarah Sad
Shibli, Rida Ahmad
al-Hawamidah, Fayha M.
Source
The Libyan Journal of Agricultural
Issue
Vol. 24, Issue 2 (31 Dec. 2019), pp.85-96, 12 p.
Publisher
University of Tripoli Faculty of Agriculture
Publication Date
2019-12-31
Country of Publication
Libya
No. of Pages
12
Main Subjects
Abstract EN
Black nightshade (Solanum nigrum L.) is a promising medicinal plant that grows wild as a weed in Libya.
Tissue culture approach was utilized for Micropropagation of this plant to insure its stable availability for phytoindustry and research.
In vitro shoot multiplication was examined by culturing nodal explants on MS media supplemented with either BAP, kinetin or TDZ at 0, 0.5, 1.0 or 1.5 mg/L plus 0.1 mg/L NAA.
For rooting, microshoots were cultured on MS media enriched with either, IBA, NAA or IAA at 0, 0.5, 1.0 or 1.5 mg/L and were added to the media to enhance microshoots rooting.
The results revealed that a satisfactory shoot multiplication was obtained when TDZ was added to MS media, and the maximum number of the new shoots per explant 15 shoot/ explant was recorded in nodal segments treated with 1.5 mg/L TDZ with an average height of 2.06 cm.
Best rooting 6.7 roots/ explant was obtained in microshoots cultured on Growth regulators –free media, While auxin- treated microshoots showed best rooting by using IBA at 1.5 mg/L as it yielded 80% rooting percentage with 4.9 root/ explant and average root length of 1.95 cm.
Moreover, all potted plantlets were successfully acclimatized, and 100% survival rate was recorded in all plants one month after being transplanted to greenhouse conditions.
American Psychological Association (APA)
Yunus, Layla Sasi& Shibli, Rida Ahmad& Tahtamuni, Riham Walid& al-Qudah, Tamarah Sad& al-Hawamidah, Fayha M.. 2019. Micropropagation of black nightshade (Solanum nigrum L.) : a promising medicinal plant in Libya. The Libyan Journal of Agricultural،Vol. 24, no. 2, pp.85-96.
https://search.emarefa.net/detail/BIM-1088834
Modern Language Association (MLA)
Yunus, Layla Sasi…[et al.]. Micropropagation of black nightshade (Solanum nigrum L.) : a promising medicinal plant in Libya. The Libyan Journal of Agricultural Vol. 24, no. 2 (2019), pp.85-96.
https://search.emarefa.net/detail/BIM-1088834
American Medical Association (AMA)
Yunus, Layla Sasi& Shibli, Rida Ahmad& Tahtamuni, Riham Walid& al-Qudah, Tamarah Sad& al-Hawamidah, Fayha M.. Micropropagation of black nightshade (Solanum nigrum L.) : a promising medicinal plant in Libya. The Libyan Journal of Agricultural. 2019. Vol. 24, no. 2, pp.85-96.
https://search.emarefa.net/detail/BIM-1088834
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-1088834