Serological and molecular detection of cowpae mosaic virus infecting cowpae in iraq
Other Title(s)
التشخيص المناعي و الجزيئي لفايروس موزائيك اللوبياء الذي يصيب نبات اللوبياء في العراق
Joint Authors
al-Shammari, Abd al-Hakim Ali
al-Kuwaiti, Nawras Abd al-Ilah
Source
Diyala Agricultural Sciences Journal
Issue
Vol. 11, Issue 1 (31 Dec. 2019), pp.9-17, 9 p.
Publisher
University of Diyala College of Agriculture
Publication Date
2019-12-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract EN
This study was conducted to detect CPMV in infecting cowpea plantes at Plant Protection Department/College of Agriculture, University of Baghdad.
Symptomatic cowpea plants collected from fields in Baghdad and Diyala Provinces were tested by Emzyme-linked immunosorbent assay (ELISA), reverse transcription- polymerase chain reaction RT-PCR and Immuno-capture reverse transcription-polymerase chain reaction (IC-RT-PCR) using commercial kits.
ELISA approach could detect Cowpea mosaic virus CpMV in tested samples but with virus concentration lower than the positive control supplied with the kit, indicating a unique virus strain.
RNA from samples showed the highest CpMV concentration were extracted by direct release and used in IC-PT-PCR using como2 a comovirus specific primer set.
cDNA was synthetized using como2 revers primer.
RT-PCR confirmed the detection of comoviruses when amplified 200 bp DNA fragments from CpMV infected samples.
Sequence analysis confirmed CpMV detection when compared with equivalent GenBank sequences.
The sequence obtained shared 94.1% maximum nucleotide identity with RNA dependent RNA polymerase (RdRp) gene of CpMV from Egypt (Acc.
No.
KT438619 and X00206).
Both sequence analysis and IC-RT-PCR indicated that virus isolate detected could be an Iraq divergent strain of CpMV.
American Psychological Association (APA)
al-Shammari, Abd al-Hakim Ali& al-Kuwaiti, Nawras Abd al-Ilah. 2019. Serological and molecular detection of cowpae mosaic virus infecting cowpae in iraq. Diyala Agricultural Sciences Journal،Vol. 11, no. 1, pp.9-17.
https://search.emarefa.net/detail/BIM-1089947
Modern Language Association (MLA)
al-Shammari, Abd al-Hakim Ali& al-Kuwaiti, Nawras Abd al-Ilah. Serological and molecular detection of cowpae mosaic virus infecting cowpae in iraq. Diyala Agricultural Sciences Journal Vol. 11, no. 1 (2019), pp.9-17.
https://search.emarefa.net/detail/BIM-1089947
American Medical Association (AMA)
al-Shammari, Abd al-Hakim Ali& al-Kuwaiti, Nawras Abd al-Ilah. Serological and molecular detection of cowpae mosaic virus infecting cowpae in iraq. Diyala Agricultural Sciences Journal. 2019. Vol. 11, no. 1, pp.9-17.
https://search.emarefa.net/detail/BIM-1089947
Data Type
Journal Articles
Language
English
Notes
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Record ID
BIM-1089947