Evaluation the phenotypic and genotypic variations for clinical isolates of arthroderma otae complex

Abstract EN

M.

canis , M.

audouinii and M.

ferrugineum are three species ofArthroderma otae com- plex, which morphologically and genetically closely related.

Conventional identification of phenotypic and biochemical characteristics are usually yielding unclear results.

Geno- typic identification by using modern technique which is yielding more accurate results.

In this study, ITS region of rDNA was sequenced and phylogenetic tree analysis for Mi- crosporum spp.

were observed three clusters of A.

otae isolates.

When sequencing identi- fies with the reference strains sequence of GenBank Database detected several mutations in multiple locus either by miss matching or genetic gaps.

By using Mega 6 software and display toggle conserved sites at the 50% level with different colors of A.

otae isolates showed identification of the colors in the nitrogenous bases alignment belong to the same species of the telomorph.