Determination of Brucella. abortus biovars infected human in the middle and Southern of Iraq

Abstract EN

Background: Brucella abortus (B.

abortus) is a zoonotic bacterium causes both health and economic losses in Iraq.

Objective: The isolation and detection of species and subspecies is very important to understand how the outbreak occurs and to improve vaccine.

Materials and methods: To determine the dominant Biovars (bv) in Iraq (150) blood samples were collected from patients suspected with brucellosis from (Baghdad, Wasit, Babil, Karbala, Al Najaf, Al-Qadisiyah, Maysan, Al-Muthanna and Al Basrah) hospital's from June 2015 to February 2016.

Serum was separated and tested using Rose Bengal Plate Test (RBPT) and Enzyme Linked Immune Sorbent Assay (ELIDA), then positive samples to ELISA were cultivated on Brucella agar.

Bacteria were tested to biotyping using both conventional methods include (CO2 requirement, urease test, H2S production, and growth on dyes), and Non-conventional methods include AMOS-ERY using set of primers (PIS711, PBa, ERY1, ERY2 and DEL569).

Results: Ninety six samples were found positive and (54) samples were found negative that tested by RBPT.

The ELISA specific for B.

abortus infected human was conducted on the positive RBPT only (38) samples were found positive and (15) samples were found negative and (43) samples were marked undetermined.

The result of biochemical showed B.

abortus bv (1, 3, 4, 5, 6 and 9) and B.

meletensis (bv2) very closed to the AMOS-ERY test results that showed groups of biovars (1, 3, 5, 6 and 9).

Conclusion: The Molecular diagnostics have proven its effectiveness in determining the Biovars of these bacteria.