Phytochemical content and the potential of punica granatum peel extracts as radical scavengers and dipeptidyl peptidase-4 inhibitors
Other Title(s)
تحليل المحتوى الكيمونباتي لمستخلصات قشور الرمان و تشخيص قابليتها على كسح الجذور الحرة و تثبيط أنزيم داي بيبتايديل بيبتايديز-4 (Dpp4)
Joint Authors
Malik, Latif Abd al-Kazim
Aziz, Ghazi Munim
Adhiah, Ali Husayn
Source
Journal of Biotechnology Research Center
Issue
Vol. 13, Issue 1 (30 Jun. 2019), pp.5-11, 7 p.
Publisher
al-Nahrain University Biotechnology Research Center
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Topics
- Chemistry
- Activities
- Roots
- Natural products
- Gallic acid
- Pomegranate
- Content analysis
- Dipeptidyl peptidase-4
- Peels(Plants)
- Distilled water
- Ellagic acid
Abstract EN
Background: Natural products of herbal medicines and their secondary metabolites have been suggested to treat T2DM and they may have the potential as DPP-4 inhibitors.
Objective: This study was conducted to estimate the phytochemical content, radical scavenging activity (RSA) and DPP-4 (dipeptidyl peptidase-4) relative inhibition activity (RIA) of different extracts of Punica granatum peel.
Material and Methods: Fruit peel of pomegranate plant Punica granatum were subjected to extraction with four solvents (distilled water(AQ), 80% methanol(ME), 80% acetone(AC) and a mixed solvent(MX) that included methanol, ethanol, acetone and n-butanol at proportions (7: 1: 1: 1), respectively.
Yielded extracts were phytochemically analyzed for total polyphenols (TP), flavonoids (TF) and HPLC analysis.
The DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging activity (RSA) and DPP-4 (dipeptidyl peptidase-4) relative inhibition activity (RIA) were also assessed for each extract.
Results: The results showed that acetone solvent (AC) extract of fruit peel of Punica granatum recorded the highest content of TP and TF (159.9 ± 1.0 mg Gallic acid equivalent/g dry mass) and (7.6 ± 0.2 mg catechin equivalent/g dry mass, respectively).
Mixture extract (MX) was showed the highest antioxidant activity of (54.7 ± 0.9% RSA).
While methanol extract (ME) recorded the highest DPP-4 RIA (47.1 ± 1.5% ).
HPLC analysis showed the presence of Gallic acid, Tannic acid, Ellagic acid and B carboxylic acid at concentrations of (94.61, 130.14, 528.46 and 159.64 ug/ml, respectively).
Conclusion: The importance of natural products as radical scavengers and DPP-4 inhibitors is encouraged, and such biological effects were dependent on the plant species and the solvent of extraction.
Punica granatum mixture extract was suggested to have a prominent RSA, while methanol extraction of Punica granatum peels are recommended to be a target for investigations involved in the development of anti-T2DM and anti-cancer therapies.
American Psychological Association (APA)
Malik, Latif Abd al-Kazim& Adhiah, Ali Husayn& Aziz, Ghazi Munim. 2019. Phytochemical content and the potential of punica granatum peel extracts as radical scavengers and dipeptidyl peptidase-4 inhibitors. Journal of Biotechnology Research Center،Vol. 13, no. 1, pp.5-11.
https://search.emarefa.net/detail/BIM-1093933
Modern Language Association (MLA)
Malik, Latif Abd al-Kazim…[et al.]. Phytochemical content and the potential of punica granatum peel extracts as radical scavengers and dipeptidyl peptidase-4 inhibitors. Journal of Biotechnology Research Center Vol. 13, no. 1 (2019), pp.5-11.
https://search.emarefa.net/detail/BIM-1093933
American Medical Association (AMA)
Malik, Latif Abd al-Kazim& Adhiah, Ali Husayn& Aziz, Ghazi Munim. Phytochemical content and the potential of punica granatum peel extracts as radical scavengers and dipeptidyl peptidase-4 inhibitors. Journal of Biotechnology Research Center. 2019. Vol. 13, no. 1, pp.5-11.
https://search.emarefa.net/detail/BIM-1093933
Data Type
Journal Articles
Language
English
Notes
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Record ID
BIM-1093933