PCR detection of toxoplasma gondii B1 gene in women suffering from abortion
Other Title(s)
كشف تفاعل البوليمريز المتسلسل لجين B1 في داء المقوسات في النساء اللواتي يعانين من الإجهاض
Joint Authors
Mahmud, Asil Shakir
al-Saray, Sabihah Abd al-Husayn
al-Qazzaz, Abd al-Karim Abd al-Razzaq Abd al-Wahhab
Source
Journal of Biotechnology Research Center
Issue
Vol. 13, Issue 1 (30 Jun. 2019), pp.12-15, 4 p.
Publisher
al-Nahrain University Biotechnology Research Center
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
4
Main Subjects
Topics
Abstract EN
Background: Primary infection of maternal with toxoplasmosis during gestation and this infection transmission to the fetus continue to be the cause complex disease in offspring.
Objective: This study was conducted to test the utility of nested Polymerase Chain Reaction (nPCR) assay to detect recent infections with Toxoplasma in abortive women.
Material and methods: Toxoplasma gondii DNA was detected by using B1 gene as a target for amplification which was highly specific for T.
gondii and is well conserved among all of the tested strains.
Blood from 60 abortive women and 25 apparently healthy pregnant women with no history of abortion (as control group) were taken in this current study.
Results: The results revealed that nPCR was positive in 48(80% ) subjects and negative in 12(20% ), Chi-square- χ2 for patients and control was ( 13.82, 15.75 ) respectively.
Conclusion: It can be concluded that nPCR assay in blood has advantage in detection of recent and active toxoplasmosis.
American Psychological Association (APA)
Mahmud, Asil Shakir& al-Saray, Sabihah Abd al-Husayn& al-Qazzaz, Abd al-Karim Abd al-Razzaq Abd al-Wahhab. 2019. PCR detection of toxoplasma gondii B1 gene in women suffering from abortion. Journal of Biotechnology Research Center،Vol. 13, no. 1, pp.12-15.
https://search.emarefa.net/detail/BIM-1093934
Modern Language Association (MLA)
al-Qazzaz, Abd al-Karim Abd al-Razzaq Abd al-Wahhab…[et al.]. PCR detection of toxoplasma gondii B1 gene in women suffering from abortion. Journal of Biotechnology Research Center Vol. 13, no. 1 (2019), pp.12-15.
https://search.emarefa.net/detail/BIM-1093934
American Medical Association (AMA)
Mahmud, Asil Shakir& al-Saray, Sabihah Abd al-Husayn& al-Qazzaz, Abd al-Karim Abd al-Razzaq Abd al-Wahhab. PCR detection of toxoplasma gondii B1 gene in women suffering from abortion. Journal of Biotechnology Research Center. 2019. Vol. 13, no. 1, pp.12-15.
https://search.emarefa.net/detail/BIM-1093934
Data Type
Journal Articles
Language
English
Notes
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Record ID
BIM-1093934