Leishmanicidal activity of artemisinin against cutaneous leishmaniasis, in vitro
Other Title(s)
فعالية الأرتميسينين المضادة للشمانيا الجلدية خارج الجسم الحي
Joint Authors
Hassan, Ghufran Muhammad
Ali, Haydar Zuhayr
Source
Journal of Biotechnology Research Center
Issue
Vol. 13, Issue 1 (30 Jun. 2019), pp.16-22, 7 p.
Publisher
al-Nahrain University Biotechnology Research Center
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Topics
Abstract EN
Background: Cutaneous leishmaniasis (CL) is a neglected disease in tropical countries, including Iraq.
Several studies have sought to examine chemotherapies for leishmaniasis treatment but most of them are of toxic and/or undesirable side effect, therefore, the need for investigating new fewer toxic therapies is essential.
Aim of study: In this study, the cytotoxic effect of Artemisinin (ART), a novel herbal compound, was screened against the two forms, promastigotes and amastigotes, of the Iraqi isolate of Leishmania tropica, the causative agent of Baghdad boil.
Material and methods: Different concentrations (1000, 500, 250, 125, 62.5, 31.25, 15.6 and 7.8) µM of Artemisinin were screened to investigate the leishmanicidal activity of the herbal compound against the two forms of the parasite along three times of follow up (24, 48, 72) hour using MTT cytotoxicity assay.
Results: The results showed that growth rate and cell viability were significantly decreased at all studied concentrations.
The IC50 was measured after 72 hours of follow up and was 2.625 µM and 2.636 µM for promastigotes and amastigotes, respectively.
Conclusion: These findings approved the leishmanicidal efficacy of Artemisinin against the of L.
tropica and can be further studied to screen its effectiveness in vivo for exploring a safer herbal drug for treatment of cutaneous leishmaniasis.
American Psychological Association (APA)
Hassan, Ghufran Muhammad& Ali, Haydar Zuhayr. 2019. Leishmanicidal activity of artemisinin against cutaneous leishmaniasis, in vitro. Journal of Biotechnology Research Center،Vol. 13, no. 1, pp.16-22.
https://search.emarefa.net/detail/BIM-1093935
Modern Language Association (MLA)
Hassan, Ghufran Muhammad& Ali, Haydar Zuhayr. Leishmanicidal activity of artemisinin against cutaneous leishmaniasis, in vitro. Journal of Biotechnology Research Center Vol. 13, no. 1 (2019), pp.16-22.
https://search.emarefa.net/detail/BIM-1093935
American Medical Association (AMA)
Hassan, Ghufran Muhammad& Ali, Haydar Zuhayr. Leishmanicidal activity of artemisinin against cutaneous leishmaniasis, in vitro. Journal of Biotechnology Research Center. 2019. Vol. 13, no. 1, pp.16-22.
https://search.emarefa.net/detail/BIM-1093935
Data Type
Journal Articles
Language
English
Notes
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Record ID
BIM-1093935