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Molecular serotyping of cryptococcus neoformans isolated from environmental sources
Joint Authors
Mujaybl, Fatimah A.
Majid, Nabil S.
Source
Journal of Babylon University : Journal of Applied and Pure Sciences
Issue
Vol. 26, Issue 2 (28 Feb. 2018), pp.315-325, 11 p.
Publisher
Publication Date
2018-02-28
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Abstract EN
is also, aimed to determine the molecular serotyping among different environmental resources samples of Cryptococcus neoformans, by using phenotypic and molecular techniques.
So, this study is designed to investigate the molecular serotyping of C.
neoformans at a genetic level in order to identify the serotypes belong to this yeast and to differentiate them from each other.
To achieve these goals, environmental resources samples were collected from different area of Najaf province during (2015).
Culture tests are done for samples in The same aspect includes identification by molecular methods.
The polymerase chain reaction method (PCR) is used to identify the Cryptococcus serotype genetically.
Depending on amplification of genomic DNA, the entire isolates are diagnosed as Cryptococcus yeasts.
However, the most recurring species are the C.
neoformans that rated 80%, while the C.
gattii that rated 20% is the less recurring one.
The results of amplifing the genomic DNA, using specific primer, show bands with molecular weight that is (387 bp) approximately for 12 isolates.
However, the other species showed band with molecular weight that was (359 bp).
The isolates are diagnosed genetically using the RFLP – PCR method as restriction enzyme with (Bsm F1, Hpa II) through which systematic important information appears for the genetic relations in the studied isolates that are related for two types.
PCR assay revealed that five different serotypic patterns are detected as: serotype A ,serotype B , serotype C , serotype D and serotype AD .It can be concluded that the PCR targeting the genomic DNA and the REFP – PCR are rapid and simple techniques for serotyping C.
neoformans and are useful not only for discrimination of C.
neoformans from its related species, but also for epidemiological study at the molecular level.
This study provides the detection genetically serotyping of C.
neoformans which is isolated from different environmental resources in Najaf province
American Psychological Association (APA)
Mujaybl, Fatimah A.& Majid, Nabil S.. 2018. Molecular serotyping of cryptococcus neoformans isolated from environmental sources. Journal of Babylon University : Journal of Applied and Pure Sciences،Vol. 26, no. 2, pp.315-325.
https://search.emarefa.net/detail/BIM-1094049
Modern Language Association (MLA)
Mujaybl, Fatimah A.& Majid, Nabil S.. Molecular serotyping of cryptococcus neoformans isolated from environmental sources. Journal of Babylon University : Journal of Applied and Pure Sciences Vol. 26, no. 2 (2018), pp.315-325.
https://search.emarefa.net/detail/BIM-1094049
American Medical Association (AMA)
Mujaybl, Fatimah A.& Majid, Nabil S.. Molecular serotyping of cryptococcus neoformans isolated from environmental sources. Journal of Babylon University : Journal of Applied and Pure Sciences. 2018. Vol. 26, no. 2, pp.315-325.
https://search.emarefa.net/detail/BIM-1094049
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 325
Record ID
BIM-1094049