In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes
Joint Authors
Nolan, Jacqueline
Sarimollaoglu, Mustafa
Nedosekin, Dmitry A.
Jamshidi-Parsian, Azemat
Galanzha, Ekaterina I.
Kore, Rajshekhar A.
Zharov, Vladimir P.
Griffin, Robert J.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-14
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Circulating tumor cells (CTCs) demonstrated the potential as prognostic markers of metastatic development.
However, the incurable metastasis can already be developed at the time of initial diagnosis with the existing CTC assays.
Alternatively, tumor-associated particles (CTPs) including exosomes can be a more valuable prognostic marker because they can be released from the primary tumor long before CTCs and in larger amount.
However, little progress has been made in high sensitivity detection of CTPs, especially in vivo.
We show here that in vivo integrated photoacoustic (PA) and fluorescence flow cytometry (PAFFC) platform can provide the detection of melanoma and breast-cancer-associated single CTPs with endogenously expressed melanin and genetically engineered proteins or exogenous dyes as PA and fluorescent contrast agents.
The two-beam, time-of-light PAFFC can measure the sizes of CTCs and CTPs and identify bulk and rolling CTCs and CTC clusters, with no influence on blood flow instability.
This technique revealed a higher concentration of CTPs than CTCs at an early cancer stage.
Because a single tumor cell can release many CTPs and in vivo PAFFC can examine the whole blood volume, PAFFC diagnostic platform has the potential to dramatically improve (up to 105-fold) the sensitivity of cancer diagnosis.
American Psychological Association (APA)
Nolan, Jacqueline& Sarimollaoglu, Mustafa& Nedosekin, Dmitry A.& Jamshidi-Parsian, Azemat& Galanzha, Ekaterina I.& Kore, Rajshekhar A.…[et al.]. 2016. In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes. Analytical Cellular Pathology،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1094956
Modern Language Association (MLA)
Nolan, Jacqueline…[et al.]. In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes. Analytical Cellular Pathology No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1094956
American Medical Association (AMA)
Nolan, Jacqueline& Sarimollaoglu, Mustafa& Nedosekin, Dmitry A.& Jamshidi-Parsian, Azemat& Galanzha, Ekaterina I.& Kore, Rajshekhar A.…[et al.]. In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes. Analytical Cellular Pathology. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1094956
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1094956