Annexin A3 Knockdown Suppresses Lung Adenocarcinoma
Joint Authors
Liu, Ying-Fu
Liu, Qing-Qing
Zhang, Yue-Hua
Qiu, Jing-Hua
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Our previous study identified an elevated abundance of annexin A3 (Anxa3) as a novel prognostic biomarker of lung adenocarcinoma (LADC) through quantitative proteomics analysis.
However, the biological functions of Anxa3 in LADC are not fully clear.
In this study, in vitro and in vivo assays were performed to investigate the effects of Anxa3 downregulation on the growth, migration, invasion, metastasis, and signaling pathway activation of LADC cells.
After Anxa3 downregulation, the growth of A549 and LTEP-a2 LADC cells was slowed and they showed decreased migration and invasion in vitro.
Anxa3 knockdown significantly inhibited tumor formation by A549 cells in vivo; while many metastases were formed by control A549 cells, there were obvious reductions in the numbers of lung, liver, and brain metastases formed by Anxa3 knockdown in A549 cells.
Furthermore, Anxa3 knockdown significantly decreased MMP-2 and N-cadherin expression and increased E-cadherin expression both in cell lines in vitro and in tumor nodules examined during in vivo tumorigenesis assays.
Interestingly, Anxa3 downregulation reduced the phosphorylated levels of MEK and ERK.
In summary, Anxa3 knockdown inhibited the growth, migration, invasion, and metastasis of LADC, decreased the activation of the MEK/ERK signaling pathway, and modulated the expression of MMP-2, E-cadherin, and N-cadherin.
American Psychological Association (APA)
Liu, Ying-Fu& Liu, Qing-Qing& Zhang, Yue-Hua& Qiu, Jing-Hua. 2016. Annexin A3 Knockdown Suppresses Lung Adenocarcinoma. Analytical Cellular Pathology،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1094962
Modern Language Association (MLA)
Liu, Ying-Fu…[et al.]. Annexin A3 Knockdown Suppresses Lung Adenocarcinoma. Analytical Cellular Pathology No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1094962
American Medical Association (AMA)
Liu, Ying-Fu& Liu, Qing-Qing& Zhang, Yue-Hua& Qiu, Jing-Hua. Annexin A3 Knockdown Suppresses Lung Adenocarcinoma. Analytical Cellular Pathology. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1094962
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1094962