Image Montaging for Creating a Virtual Pathology Slide: An Innovative and Economical Tool to Obtain a Whole Slide Image
Joint Authors
Chippagiri, Prashanthi
Pandurangappa, Rohit
Annavajjula, Saileela
Rajashekaraiah, Premalatha Bidadi
Banavar Ravi, Spoorthi
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Microscopes are omnipresent throughout the field of biological research.
With microscopes one can see in detail what is going on at the cellular level in tissues.
Though it is a ubiquitous tool, the limitation is that with high magnification there is a small field of view.
It is often advantageous to see an entire sample at high magnification.
Over the years technological advancements in optics have helped to provide solutions to this limitation of microscopes by creating the so-called dedicated “slide scanners” which can provide a “whole slide digital image.” These scanners can provide seamless, large-field-of-view, high resolution image of entire tissue section.
The only disadvantage of such complete slide imaging system is its outrageous cost, thereby hindering their practical use by most laboratories, especially in developing and low resource countries.
Methods.
In a quest for their substitute, we tried commonly used image editing software Adobe Photoshop along with a basic image capturing device attached to a trinocular microscope to create a digital pathology slide.
Results.
The seamless image created using Adobe Photoshop maintained its diagnostic quality.
Conclusion.
With time and effort photomicrographs obtained from a basic camera-microscope set up can be combined and merged in Adobe Photoshop to create a whole slide digital image of practically usable quality at a negligible cost.
American Psychological Association (APA)
Banavar Ravi, Spoorthi& Chippagiri, Prashanthi& Pandurangappa, Rohit& Annavajjula, Saileela& Rajashekaraiah, Premalatha Bidadi. 2016. Image Montaging for Creating a Virtual Pathology Slide: An Innovative and Economical Tool to Obtain a Whole Slide Image. Analytical Cellular Pathology،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1094976
Modern Language Association (MLA)
Banavar Ravi, Spoorthi…[et al.]. Image Montaging for Creating a Virtual Pathology Slide: An Innovative and Economical Tool to Obtain a Whole Slide Image. Analytical Cellular Pathology No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1094976
American Medical Association (AMA)
Banavar Ravi, Spoorthi& Chippagiri, Prashanthi& Pandurangappa, Rohit& Annavajjula, Saileela& Rajashekaraiah, Premalatha Bidadi. Image Montaging for Creating a Virtual Pathology Slide: An Innovative and Economical Tool to Obtain a Whole Slide Image. Analytical Cellular Pathology. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1094976
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1094976