Evaluation of HLA-G 14 bp InsDel and +3142G>C Polymorphism with Susceptibility and Early Disease Activity in Rheumatoid Arthritis

Abstract EN

Purpose/Background.

Mounting evidence designates that HLA-G plays a role in the regulation of inflammatory processes and autoimmune diseases.

There are controversial reports concerning the impact of HLA-G gene polymorphism on rheumatoid arthritis (RA).

This study was aimed at examining the impact of 14 bp ins/del and +3142G>C polymorphism with susceptibility and early disease activity in RA patients in a sample of the Iranian population.

Methods.

This case-control study was done on 194 patients with RA and 158 healthy subjects.

The HLA-G rs1063320 (+3142G>C) and rs66554220 (14 bp ins/del) variants were genotype by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFP) and PCR method, respectively.

Results.

The HLA-G +3142G>C polymorphism significantly decreased the risk of RA in codominant (OR = 0.61, 95% CI = 0.38–0.97, p = 0.038 , GC versus GG; OR = 0.36, 95% CI = 0.14–0.92, p = 0.034 , CC versus GG), dominant (OR = 0.56, 95% CI = 0.36–0.87, p = 0.011 , GC + CC versus GG), and allele (OR = 0.58, 95% CI = 0.41–0.84, p = 0.004 , C versus G) inheritance models tested.

Our finding did not support an association between HLA-G 14 bp ins/del variant and risk/protection of RA.

In addition, no significant association was found between the polymorphism and early disease activity.

Conclusion.

In summary, our results showed that HLA-G +3142G>C gene polymorphism significantly decreased the risk of RA in a sample of the Iranian population.